Anti-CDY1B Polyclonal Antibody for WB, ELISA - Q9Y6F8

Core Product Specifications and Parameters

Biological Background: CDY1B Function and Localization

• CDY1B (Testis-specific chromodomain protein Y 1) is a chromatin-modifying enzyme encoded by the CDY1B gene, which belongs to the Y-linked CDY1 gene family that also includes CDY1A.
• It functions as a histone acetyltransferase (HAT) with a preference for acetylating histone H4, thereby contributing to chromatin remodeling and transcriptional regulation.
• The protein localizes to the nucleus, consistent with its role in modifying chromatin structure.
• Expression is highly testis-specific; CDY1B protein has been detected in spermatids, indicating a specialized function in post-meiotic spermatogenesis.
• Alternative splicing of the CDY1B transcript generates multiple isoforms, which may exhibit distinct regulatory properties or tissue-specific roles.
• As an acyltransferase, it belongs to the broader transferase class of enzymes and is annotated as a reference proteome component in UniProt.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 60–320 of human CDY1B, which may elicit antibodies recognizing multiple isoforms or conserved regions.
• For Western blot, the predicted molecular weight is approximately 60 kDa; consider using appropriate loading controls and confirm band size in testis-derived or transfected cell lysates.
• Due to the polyclonal nature, additional bands corresponding to alternative splice variants may be observed; validate specificity in the relevant sample system.
• The antibody cross-reacts with mouse and rat, enabling potential use in rodent models; however, confirm reactivity empirically for your experimental conditions.
• For ELISA, the antibody can be used as a capture or detection reagent; titrate and validate with purified CDY1B protein or known positive samples to establish assay conditions.
• Because the target is testis-specific and expressed at low levels, enrichment of spermatid populations or use of testis tissue is recommended for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides researchers and diagnostic manufacturers with streamlined access to CDY1B antibodies and complementary chromatin biology reagents. Our offerings extend to validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare testis-specific epigenetic targets—supporting your workflow from early R&D to clinical applications.