Anti-CDX2 Rabbit Monoclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - Q99626

Core Product Specifications and Parameters

Biological Background: CDX2 Function and Localization

• CDX2 is a homeobox transcription factor, also called CDX-3 or caudal-type homeobox protein 2, that plays a critical role in intestinal development and differentiation.
• It binds to specific DNA sequences in the promoters of target genes such as sucrase-isomaltase (SI), vitamin D receptor (VDR), lactase-phlorizin hydrolase (LPH), claudin-2 (CLDN2), mucin-2 (MUC2), and carbonic anhydrase 1 (CA1), thereby activating their transcription (By similarity).
• CDX2 preferentially binds to methylated DNA, as demonstrated in human studies, indicating a role in epigenetic regulation. Related references: PMID:28473536
• It is a member of the homeobox gene family and contains a conserved homeodomain DNA-binding motif.
• Localization is exclusively nuclear, consistent with its function as a transcriptional regulator.
• Post-translational modifications include phosphorylation and ubiquitin conjugation (based on keywords), which may modulate its activity and stability.
• CDX2 expression is detected in small intestine, colon, and pancreas, underscoring its tissue-specific regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 1–100) targets the N-terminal region of CDX2; consider that this domain may be conserved across species, contributing to the observed cross-reactivity.
• For western blot, a band at approximately 34 kDa is expected; however, phosphorylation or other modifications may cause minor mobility shifts—validate in the relevant sample system.
• This antibody is suitable for multiple applications including WB, IP, IF-P, IHC-P, and ELISA; optimal working concentrations should be determined experimentally for each assay.
• Cross-reactivity with mouse and rat is reported; confirm performance in the intended species and tissue context before large-scale studies.
• For IHC-P, nuclear localization of CDX2 serves as a useful internal control; optimize fixation and antigen retrieval protocols to preserve antigenicity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined sourcing solution for IVD raw materials, including validated antibodies, bulk reagents, and technical support. This anti-CDX2 rabbit monoclonal antibody supports gastrointestinal and oncology research, and CamelBio can further assist with custom antibody development and rare-target sourcing to meet your project needs.