Anti-CDKN3 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16667

Core Product Specifications and Parameters

Biological Background: CDKN3 Function and Localization

• CDKN3 (Cyclin-dependent kinase inhibitor 3), also known as KAP, CDI1, CIP2, KAP1, is a dual-specificity protein phosphatase that belongs to the protein tyrosine phosphatase family.
• Functions as a cyclin-dependent kinase (CDK) inhibitor, dephosphorylating CDK2 at Thr-160 in a cyclin-dependent manner, thereby regulating cell cycle progression. Related references: PMID:7569954
• Exhibits dual specificity, capable of dephosphorylating substrates containing phosphotyrosine or phosphoserine residues. Related references: PMID:8127873, PMID:8242750
• Localizes to the cytoplasm, particularly the perinuclear region.
• Associated with disease variants and plays a role in cell cycle control.
• Alternative splicing generates multiple isoforms of the protein.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein spanning the N-terminal 1-69 amino acids of human CDKN3, ensuring high specificity for the target.
• For Western blot (WB), the predicted molecular weight is approximately 24 kDa; validate detection in Human and Mouse lysates, as cross-reactivity is documented.
• For immunohistochemistry (IHC-P), immunofluorescence (IF/ICC), and ELISA, consider optimizing blocking and antibody dilution conditions using appropriate positive and negative controls.
• Non-validated applications or species should be tested empirically with proper controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined, end-to-end supply solution for IVD raw materials, technical services, and consulting, assisting diagnostic manufacturers and research institutes from concept to clinical application. For cell cycle and phosphatase-related projects, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and bespoke sourcing of rare-target raw materials. Contact us to integrate this high-quality anti-CDKN3 antibody and other critical components into your development pipeline.