Anti-CDKN2C/p18-INK4C Rabbit Monoclonal Antibody for WB - P42773

Core Product Specifications and Parameters

Biological Background: CDKN2C/p18-INK4C Function and Localization

• CDKN2C, also known as cyclin-dependent kinase 4 inhibitor C, p18-INK4c, or p18-INK6, is a member of the INK4 family of CDK inhibitors.
• The protein functions as a potent inhibitor of CDK6 and, to a lesser extent, CDK4, thereby regulating cell cycle progression.
• By inhibiting CDK4/6, p18-INK4C prevents phosphorylation of the retinoblastoma protein (RB), leading to cell cycle arrest in G1 phase.
• p18-INK4C contains ankyrin repeat motifs, which are essential for its protein–protein interactions and inhibitory function.
• Highest expression is observed in skeletal muscle, with additional presence in pancreas and heart tissues.
• The protein plays a critical role in controlling cell proliferation and is implicated in tumor suppression.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1–120 of human CDKN2C) corresponds to the N-terminal region, which is expected to be conserved across species, supporting cross-reactivity with mouse and rat.
• For Western blot, a band at approximately 18 kDa (observed molecular weight) should be detected; consider using appropriate positive controls such as skeletal muscle or pancreas lysates.
• In immunofluorescence/ICC, optimize fixation and permeabilization conditions to preserve epitope recognition.
• For ELISA, pair with a matched detection antibody to enhance specificity; validate with recombinant protein or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we empower diagnostic developers with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-CDKN2C/p18-INK4C clone, ideal for cell cycle and oncology research applications. Our one-stop sourcing model covers validated antibody pairs, bulk ancillary reagents, and custom rare-target development, streamlining your path from concept to clinic. Partner with us to secure reliable, high-performance reagents for your diagnostic kit manufacturing.