Anti-CDKN2C/p18-INK4C Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P42773

Core Product Specifications and Parameters

Biological Background: CDKN2C/p18-INK4C Function and Localization

• CDKN2C (also known as p18-INK4c or p18-INK6) is a member of the INK4 family of cyclin-dependent kinase inhibitors, encoded by the CDKN2C gene.
• It functions primarily by interacting strongly with CDK6 and weakly with CDK4, thereby inhibiting kinase activity and blocking cell cycle progression.
• This inhibition leads to suppressed cell growth and proliferation, with a dependence on the endogenous retinoblastoma protein (RB).
• The protein structure includes multiple ANK repeats, a hallmark of the INK4 inhibitor family that mediates protein-protein interactions.
• Highest tissue expression is observed in skeletal muscle, with additional presence in the pancreas and heart.
• UniProt keywords highlight its roles in cell cycle regulation, its 3D structural characterization, and its identification through proteomics, marking it as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen used (recombinant full-length human CDKN2C) suggests the antibody may recognize native and denatured forms, supporting multiple applications.
• For Western blotting, expect a band at approximately 18 kDa; note that post-translational modifications or sample conditions may cause slight shifts.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate the antibody on known positive tissues (e.g., skeletal muscle) and consider antigen retrieval optimization.
• Given the cross-reactivity with mouse and rat, this antibody can be employed in studies using these common model organisms.
• ELISA performance may require titration and blocking conditions specific to your assay format; recombinant protein can serve as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, providing this high-quality anti-CDKN2C/p18-INK4C polyclonal antibody for cell cycle and cancer research. Our offerings include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, supporting your diagnostic development from concept to clinic. Let us streamline your supply chain and accelerate your project timelines with reliable, research-backed materials.