Anti-CDKN2B Rabbit Polyclonal Antibody for WB, ELISA - P42772

Core Product Specifications and Parameters

Biological Background: Cyclin-dependent kinase 4 inhibitor B Function and Localization

• CDKN2B encodes cyclin-dependent kinase 4 inhibitor B (also known as p15INK4b, MTS2), a member of the INK4 family of CDK inhibitors.
• It acts as a potent inhibitor of CDK4 and CDK6, thereby regulating the G1/S transition of the cell cycle.
• CDKN2B is implicated in TGF-beta-induced cell cycle arrest, serving as a potential effector of this pathway.
• The protein localizes predominantly to the cytoplasm (UniProt annotation).
• Alternative splicing yields multiple isoforms; isoform 2 is expressed in normal keratinocytes, fibroblasts, and tumor cell lines.
• The protein contains ANK repeats and is classified as a tumor suppressor, with roles in cell cycle control and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 1-138 of human CDKN2B, likely targeting the full-length protein. For Western blotting, a band around 15 kDa is expected.
• Optimize antibody dilution and blocking conditions using appropriate positive controls, such as cell lines expressing CDKN2B.
• In ELISA, consider using the antibody for capture or detection of CDKN2B in cell lysates or recombinant protein samples; validate specificity with negative controls.
• Cross-reactivity with mouse and rat indicates potential use in these species, but confirm reactivity in your specific assay system.
• Since CDKN2B functions as a tumor suppressor, consider using relevant cancer cell models to study its expression and regulation.

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CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibodies, antigens, and ancillary reagents to support diagnostic development from concept to clinic. Our CDKN2B rabbit polyclonal antibody and other cell cycle-related products are ideal for advancing cancer diagnostic assays and research. Explore our comprehensive portfolio or contact us for custom sourcing solutions.