Anti-CDKN2A/p19ARF Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q64364-1

Core Product Specifications and Parameters

Biological Background: CDKN2A/p19ARF Function and Localization

• CDKN2A/p19ARF is annotated as the mouse tumor suppressor ARF isoform, part of the Cdkn2a gene locus.
• The Cdkn2a gene generates multiple isoforms, including p19ARF and p16INK4a, through alternative reading frames.
• The protein is known under several aliases: Alternative reading frame (ARF), Cyclin-dependent kinase inhibitor 2A, and others.
• Its calculated molecular weight is 19,238 Da, consistent with an observed band around 19 kDa.
• No specific subcellular localization, tissue expression, or post-translational modifications are provided in the source record.
• While tumor suppressor ARF proteins classically interact with MDM2 to stabilize p53, such functional details are not explicitly noted in the provided entry.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 1-100 of mouse p19ARF; thus, the antibody likely recognizes the N-terminal region. For IF applications, consider that the epitope may be masked in native protein complexes; if signal is weak, testing with denaturing pre-treatments could be informative.
• In WB, the antibody detects a band around 19 kDa under reducing conditions; validate specificity in mouse cell or tissue lysates expressing p19ARF.
• For ELISA, you may need to optimize coating conditions and use a matched antibody pair; confirm reactivity with recombinant protein or peptide.
• Cross-reactivity is reported for mouse only; if working with other species, sequence alignment of the immunogen region is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop provider of IVD raw materials, supporting diagnostic developers with validated antibodies, antigen pairs, and ancillary reagents. For research in tumor biology and cell cycle regulation, we offer a range of monoclonal and polyclonal antibodies against CDKN2A/p19ARF and related targets. Our sourcing capabilities include bulk supply, customized antibody development, and access to rare targets, ensuring seamless progression from concept to clinical application.