Anti-CDKN2A/p19ARF Rabbit Monoclonal Antibody for WB, ELISA - Q64364

Core Product Specifications and Parameters

Biological Background: CDKN2A/p19ARF (Tumor suppressor ARF) Function and Localization

• CDKN2A encodes two distinct proteins through alternative splicing: p16INK4a and p19ARF (the tumor suppressor ARF). The p19ARF protein is unrelated to p16INK4a in amino acid sequence.
• p19ARF acts as a potent tumor suppressor by inducing cell cycle arrest in G1 and G2 phases. Related references: PMID:8521522 PMID:9393858 PMID:15601844 PMID:17936562
• It binds to MDM2 and sequesters it in the nucleolus, blocking p53 degradation and enhancing p53-dependent transactivation and apoptosis. Related references: PMID:10359817 PMID:9529248
• p19ARF also induces G2 arrest and apoptosis in a p53-independent manner by preventing activation of cyclin B1/CDC2 complexes. Related references: PMID:15361884
• It down-regulates BCL6-induced transcriptional repression by binding to BCL6. Related references: PMID:15567177
• Subcellular localization: mainly found in the nucleolus and nucleoplasm; a short-lived mitochondrial isoform exists.
• p19ARF is involved in nucleolar functions, such as inhibiting ribosome biogenesis by interacting with TTF1 and promoting NPM1 degradation. Related references: PMID:20513429
• Additional functions include sumoylation regulation, interaction with E2F1 and MYC, and a role in autophagy and caspase-independent cell death.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from amino acids 1-100 of mouse p19ARF. The antibody likely recognizes the N-terminal region of the protein.
• For Western blot (WB), test the antibody on mouse cell lines known to express p19ARF. The predicted molecular weight is ~19 kDa, but additional bands may appear due to post-translational modifications or alternative isoforms. A positive control sample expressing p19ARF is recommended.
• For ELISA, the immunizing peptide can serve as a positive control. Validate the assay with known concentrations of the peptide to establish standard curves.
• The antibody cross-reactivity is reported for mouse. If working with other species, verify reactivity experimentally.
• An ice bag shipping condition is required; store the antibody at -20°C or as recommended upon receipt.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to streamline IVD raw-material procurement from concept to clinic. This CDKN2A/p19ARF rabbit monoclonal antibody supports cancer and cell cycle research, and we can further provide validated antibody pairs, bulk monoclonal antibodies, and ancillary reagents tailored to your immunoassay development needs. Whether you require rare target sourcing or comprehensive custom production, CamelBio delivers integrated supply solutions to accelerate your diagnostic projects.