Anti-CDKN2A/p16INK4a Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P42771

Core Product Specifications and Parameters

Biological Background: CDKN2A/p16INK4a Function and Localization

• CDKN2A encodes the cyclin-dependent kinase inhibitor 2A, also known as p16INK4a. It is a key tumor suppressor that negatively regulates cell cycle progression by inhibiting CDK4 and CDK6, preventing phosphorylation of the retinoblastoma protein and thereby blocking G1/S transition.
• p16INK4a is a member of the INK4 family of CDK inhibitors and contains four ankyrin repeat motifs essential for its interaction with CDKs.
• Subcellular localization includes both the cytoplasm and the nucleus, reflecting its dynamic role in cell cycle regulation.
• Widely expressed in most tissues, but notably absent in brain and skeletal muscle; a pancreas-specific isoform (isoform 3) has been identified.
• Post-translational modifications such as acetylation and phosphorylation modulate p16INK4a stability and function.
• Genetic alterations in CDKN2A are linked to various cancers, including melanoma and Li-Fraumeni syndrome, underscoring its importance as a tumor suppressor.
• The protein has a predicted molecular weight of approximately 16.5 kDa (UniProt) and is detected around 17 kDa under experimental conditions.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 57-156 of human CDKN2A/p16INK4a, covering a region that includes key ankyrin repeat domains; therefore, it is likely to recognize the native protein in multiple applications.
• For Western blotting, a band at approximately 17 kDa is expected; consider using lysates from cell lines with varying p16INK4a expression or knockout controls to confirm specificity (the product is KO validated).
• In immunofluorescence/ICC, anticipate staining in both nuclear and cytoplasmic compartments; appropriate fixation and permeabilization protocols should be validated for your specific sample system.
• ELISA performance should be assessed using recombinant p16INK4a or peptide antigens; the antibody may be suitable for sandwich immunoassay development when paired with appropriate capture and detection reagents.
• Cross-reactivity has been demonstrated for human p16INK4a; reactivity with other species or isoforms should be verified experimentally if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined pathway from concept to clinic by supplying validated IVD raw materials, including high-quality antibodies like this CDKN2A/p16INK4a monoclonal antibody. We support cell cycle and oncology research with a range of optimized reagents, from antibody pairs for ELISA development to bulk ancillary reagents. For rare target or custom needs, our technical sourcing expertise ensures reliable and scalable supply.