Anti-CDKN1C/p57 Kip2 Polyclonal Antibody for WB, ELISA - P49918

Core Product Specifications and Parameters

Biological Background: CDKN1C/p57 Kip2 Function and Localization

• CDKN1C/p57 Kip2 is a cyclin-dependent kinase inhibitor 1C, a potent tight-binding inhibitor that negatively regulates cell proliferation by targeting multiple G1 cyclin/CDK complexes.
• It strongly inhibits cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2, and to a lesser extent the mitotic cyclin B-CDC2, thereby enforcing cell cycle arrest at the G1/S transition.
• The protein is thought to contribute to the maintenance of the non-proliferative state throughout life, playing a critical role in terminal differentiation and senescence.
• Subcellularly, CDKN1C localizes predominantly to the nucleus, consistent with its direct inhibition of nuclear cyclin-dependent kinases.
• It is widely expressed across tissues, with particularly high levels in placenta and detectable levels in heart, brain, lung, skeletal muscle, kidney, pancreas, testis, and eye; liver expression is relatively low.
• Post-translational modifications include phosphorylation, and the gene is subject to methylation, as indicated by UniProt keywords; these modifications may modulate protein stability and activity.
• Alternative splicing generates multiple transcript variants, which may contribute to functional diversity or tissue-specific regulation.
• Mutations or epigenetic silencing of CDKN1C are associated with Beckwith-Wiedemann syndrome (BWS) and Wilms tumor (WBS), underscoring its role as a tumor suppressor.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal 1–107 amino acids of human CDKN1C; consider verifying antibody reactivity in your target sample by comparing with the recombinant protein control.
• For Western blot, expect a band near 32 kDa; optimize running and transfer conditions and include a suitable molecular weight marker.
• Cross-reactivity with mouse and rat is reported; for other species, align the immunogen sequence to assess potential reactivity.
• In ELISA applications, titrate the antibody using the immunogen as a coating antigen to determine optimal working dilutions.
• Handle according to standard antibody storage practices; minimize freeze-thaw cycles to preserve activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive supply partner for IVD raw materials, supporting every stage from concept to clinic. This anti-CDKN1C/p57 Kip2 polyclonal antibody is suited for cell cycle and tumor suppressor research. Through our integrated platform, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets to accelerate your diagnostic development.