Anti-CDKN1B/p27KIP1 Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P46527

Core Product Specifications and Parameters

Biological Background: CDKN1B/p27KIP1 Function and Localization

• CDKN1B encodes cyclin-dependent kinase inhibitor 1B, also known as p27Kip1, a key regulator of cell cycle progression, primarily mediating G1 arrest.
• It inhibits cyclin E-CDK2 and cyclin A-CDK2 complexes, thereby blocking cell cycle progression, but has little inhibitory effect on CDK2 bound to SPDYA. Related references: PMID:28666995
• p27Kip1 also interacts with cyclin D-CDK4 complexes, modulating their assembly, stability, and activation; its effect can be either inhibitory or activating depending on phosphorylation state and stoichiometry.
• Subcellular localization includes nucleus, cytoplasm, and endosomes, reflecting dynamic regulation during the cell cycle.
• Post-translational modifications, particularly phosphorylation, control p27Kip1 activity and subcellular distribution (Keywords: Phosphoprotein).
• It is expressed in all tissues tested, with highest levels in skeletal muscle and lowest in liver and kidney; protein expression confirmed in kidney. Related references: PMID:15509543 PMID:8033212
• Functionally, CDKN1B acts as a tumor suppressor, and its dysregulation is implicated in multiple cancers (Keywords: Tumor suppressor).
• The protein undergoes ubiquitin-like (Ubl) conjugation and is identified by proteomics, indicating post-translational control mechanisms.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human p27Kip1 (amino acids 1-198), encompassing known functional domains; the antibody may detect both phosphorylated and unphosphorylated forms — consider phosphatase treatment in relevant assays.
• For Western blotting, a band at approximately 22 kDa is expected; optimize antibody concentration and blocking conditions for your specific sample.
• In immunostaining (IF/ICC, IHC-P), note that p27Kip1 can localize to the nucleus, cytoplasm, or endosomes depending on cell cycle stage and modifications; validate subcellular distribution in your model system.
• As the antibody cross-reacts with mouse and rat, it is suitable for rodent studies; however, verify species-specific reactivity and background in your experimental setup.
• For ELISA, ensure appropriate coating and detection conditions; this antibody can be used for direct or indirect detection applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing bridge for IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality reagents. For cell cycle and oncology research, we offer validated antibody pairs, optimized polyclonals like this anti-CDKN1B/p27KIP1 antibody, and bulk ancillary reagents to support assay development. If you're exploring rare or challenging targets, our sourcing network can help secure customized immunoreagents for your pipeline.