Anti-CDKN1A/p21CIP1 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - P38936

Core Product Specifications and Parameters

Biological Background: CDKN1A/p21CIP1 Function and Localization

• Plays a central role in cell cycle regulation and DNA damage-induced G2 arrest, participating in both p53-dependent and p53-independent pathways (PubMed:9106657).
  Related references: PMID:9106657

• Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical substrates and blocking cell cycle progression.

• At low stoichiometry, facilitates nuclear import and assembly of cyclin D-CDK4 complexes; at higher ratios, inhibits the kinase activity of these complexes toward RB1.

• Competes with POLD3 for binding to PCNA, thereby inhibiting DNA polymerase delta and blocking DNA synthesis (PubMed:11595739).
  Related references: PMID:11595739

• Localizes to both the nucleus and cytoplasm; nuclear localization is essential for CDK inhibition and cell cycle arrest.

• Expressed in all adult tissues, with approximately 5-fold lower levels in the brain.

• Subject to multiple post-translational modifications, including phosphorylation, acetylation, and ubiquitination, which regulate stability and activity.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 1–164 amino acids of human p21, representing the full-length functional region. This polyclonal antibody may recognize multiple epitopes across the protein.
• For Western blot, expect a band around 18 kDa; use lysates from DNA‑damaged or p53-activated cells as positive controls.
• In immunohistochemistry (paraffin), antigen retrieval is required; optimize with known positive tissues such as colon or skin.
• For immunoprecipitation, validate complex formation with CDKs; a cross‑reactive band may appear due to the polyclonal nature.
• Cross‑reactivity with mouse has been confirmed, but performance in other applications or species should be verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers end‑to‑end support for diagnostic reagent developers, from concept to clinic. This anti‑CDKN1A/p21CIP1 antibody serves cell cycle and oncology research, and we can also supply matched antibody pairs, bulk ancillary reagents, and rare‑target raw materials tailored to your IVD development pipeline. Partner with us to streamline your procurement and accelerate assay development.