Anti-CDKN1A/p21CIP1 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, ELISA - P38936

Core Product Specifications and Parameters

Biological Background: CDKN1A/p21CIP1 Function and Localization

• Cyclin-dependent kinase inhibitor 1 (p21) is a potent inhibitor of cyclin-dependent kinases, crucial for controlling cell cycle progression at the G1/S and G2/M checkpoints. Related references: PMID:9106657
• It mediates p53/TP53-dependent inhibition of cellular proliferation in response to DNA damage, and also participates in p53-independent G2 arrest through CREB3L1 in specific cell types such as astrocytes and osteoblasts.
• p21 binds to and inhibits cyclin D-CDK4, cyclin E-CDK2, and cyclin A-CDK2 complexes, but at low stoichiometry, it can also facilitate the assembly and nuclear import of cyclin D-CDK4 complexes, thereby promoting RB1 phosphorylation.
• It directly inhibits DNA synthesis by competing with POLD3 for binding to proliferating cell nuclear antigen (PCNA), thereby blocking DNA polymerase delta activity. Related references: PMID:11595739
• Subcellular localization includes both the nucleus and cytoplasm, reflecting its roles in nuclear cell cycle regulation and cytoplasmic functions.
• p21 is widely expressed in all adult tissues, with approximately five-fold lower levels in the brain.
• The protein is subject to multiple post-translational modifications including acetylation, phosphorylation, and ubiquitination, which regulate its stability, localization, and function.

Experimental Guidance and Technical Tips

• For Western blot (WB) analysis, the predicted molecular weight is approximately 18 kDa; consider using a low molecular weight marker and optimizing transfer conditions.
• In immunoprecipitation (IP), validate the antibody’s efficiency with appropriate positive control lysates, such as DNA damage-treated cells expressing p21.
• For immunofluorescence/immunocytochemistry (IF/ICC), p21 exhibits both nuclear and cytoplasmic localization; ensure permeabilization and fixation conditions are suitable for both compartments.
• In ELISA applications, the antibody can be explored as a capture or detection agent; validate linearity and specificity using recombinant p21 protein or cell lysates.
• Given cross-reactivity with mouse, the antibody is suitable for mouse tissue studies; however, verify performance in your specific assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a single-source solution for IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. Our anti-CDKN1A/p21CIP1 rabbit monoclonal antibody supports cell cycle and oncology research, and we offer complementary products such as matched antibody pairs, optimized detection reagents, and bulk supply. With comprehensive technical support and custom sourcing, CamelBio bridges the gap from concept to clinic, accelerating your diagnostic development.