Anti-CDKL3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8IVW4

Core Product Specifications and Parameters

Biological Background: CDKL3 Function and Localization

• CDKL3 (Cyclin-dependent kinase-like 3) is also known as serine/threonine-protein kinase NKIAMRE, a member of the protein kinase superfamily encoded by the CDKL3 gene in humans.
• Subcellular localization studies indicate that CDKL3 is primarily found in the cytoplasm, consistent with its kinase signaling roles.
• The protein contains an ATP-binding domain and is annotated as a serine/threonine-protein kinase, suggesting it catalyzes the phosphorylation of serine and threonine residues on target proteins.
• Alternative splicing of the CDKL3 gene produces multiple isoforms, potentially contributing to functional diversity.
• CDKL3 is classified as a phosphoprotein, indicating that its activity may be regulated by post-translational modifications.
• Proteomics identification supports its expression across various cell types, though tissue-specific distribution data are limited.
• The calculated molecular weight of the canonical isoform is 67,514 Da (UniProt), though observed molecular weights may differ due to post-translational modifications or isoform expression.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody spans amino acids 1–120 of human CDKL3 (NP_001107047). This N-terminal region may be conserved across species, supporting the observed cross-reactivity with mouse and rat.
• For Western blotting, a band near 68 kDa is expected under reducing conditions. Slight variations in molecular weight can occur due to isoform expression or post-translational modifications.
• This antibody has been validated for Western blot (WB), immunohistochemistry (paraffin-embedded sections, IHC-P), and ELISA. Consider optimizing dilutions and conditions for each experimental system.
• When running IHC-P, appropriate antigen retrieval methods and blocking steps should be optimized to minimize background staining.
• As a polyclonal antibody, lot-to-lot variability may occur; it is recommended to validate in the relevant sample system before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from early development to clinical application. This anti-CDKL3 polyclonal antibody is part of our extensive catalog, targeting a cytoplasmic kinase relevant to signal transduction studies. Beyond individual antibodies, we can assist with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement to streamline your IVD development pipeline.