Anti-CDK9 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P50750

Core Product Specifications and Parameters

Biological Background: CDK9 Function and Localization

• CDK9 is a serine/threonine-protein kinase that functions as the catalytic subunit of the positive transcription elongation factor b (P-TEFb) complex, which plays a central role in transcription regulation. Related references: PMID:10574912, PMID:10757782, PMID:11145967
• P-TEFb phosphorylates the C-terminal domain (CTD) of RNA polymerase II and elongation factors DSIF and NELF, promoting productive transcription elongation. Related references: PMID:10574912, PMID:10757782, PMID:11145967, PMID:10912001, PMID:11112772, PMID:9857195
• CDK9 phosphorylates transcription factors and co-activators, including MYOD1, EP300, and the androgen receptor (AR), thereby modulating their transcriptional activity and promoter selectivity. Related references: PMID:10912001, PMID:11112772, PMID:12037670
• It regulates cytokine-inducible transcription networks by facilitating promoter recognition of transcription factors such as TNF-induced RELA/p65 and IL-6-induced STAT3. Related references: PMID:17956865, PMID:18362169
• The P-TEFb complex is involved in cotranscriptional histone modifications (e.g., H2Bub1, H3K4me3, H3K36me3) and RNA processing, linking transcription elongation with chromatin dynamics and mRNA maturation. Related references: PMID:15564463, PMID:19575011, PMID:19844166
• CDK9 also directly phosphorylates histone H1.4 at Ser-187 and KAT5, contributing to chromatin opening and histone acetyltransferase recruitment. Related references: PMID:28539972, PMID:29335245
• In complex with cyclin K, CDK9 promotes genome integrity by aiding recovery from replication stress, limiting single-stranded DNA accumulation, and preventing fork collapse. Isoform 2 may additionally function in DNA repair through interaction with KU70/XRCC6. Related references: PMID:20493174
• CDK9 is a ubiquitously expressed protein found in the nucleus and cytoplasm, localizing to PML bodies; it is subject to multiple post-translational modifications, including phosphorylation, acetylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 153–372 of human CDK9, spanning the kinase domain; this region is conserved, so the antibody may recognize multiple CDK9 isoforms. Validate isoform specificity in your experimental system if needed.
• For western blotting, a primary band near 43 kDa is expected; include molecular weight markers and suitable loading controls. Cross-reactivity with mouse and rat samples has been confirmed, but we recommend verifying optimal conditions for your specific lysates.
• When performing immunohistochemistry or immunofluorescence, optimize fixation (e.g., paraformaldehyde) and antigen retrieval (e.g., citrate buffer) for your tissue or cell type. Use appropriate secondary antibodies and counterstains.
• For ELISA, employ standard indirect protocols and titrate the antibody to determine optimal working dilution for your assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering validated antibodies, paired antibody sets, bulk ancillary reagents, and customized sourcing solutions from research to clinical application. This anti-CDK9 antibody serves transcription and cell cycle research, supporting studies into cancer, cardiovascular disease, and other pathologies. With deep expertise in rare target procurement, CamelBio streamlines your supply chain and accelerates your development pipeline.