Anti-CDK8 Rabbit Monoclonal Antibody for WB and ELISA - P49336

Core Product Specifications and Parameters

Biological Background: CDK8 Function and Localization

• CDK8 is the catalytic subunit of the Mediator complex, a large coactivator required for transcription of nearly all RNA polymerase II-dependent genes. Mediator bridges gene-specific regulatory proteins and the basal transcription machinery.
• Acts as a serine/threonine-protein kinase that phosphorylates the C-terminal domain (CTD) of RNA polymerase II, inhibiting transcription initiation complex formation. It also phosphorylates CCNH, leading to TFIIH down-regulation and transcriptional repression. Related references: PMID:10993082
• Interacts with MAML1 to hyperphosphorylate the NOTCH intracellular domain, promoting its degradation and modulating NOTCH signaling. Related references: PMID:15546612
• Recruited to selectively regulate the interferon response by phosphorylating the transcription factor STAT1 transactivation domain (By similarity).
• Localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• Post-translationally modified; alternative splicing generates multiple isoforms.
• Genetic variants of CDK8 are linked to neurodevelopmental disorders, including autism spectrum disorder and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human CDK8, which is highly conserved among mammals, supporting the observed cross-reactivity with mouse and rat.
• For Western blot, a band at approximately 53 kDa is expected. Optimize lysis buffer and sample preparation to account for CDK8’s incorporation into the large Mediator complex.
• When using ELISA, consider pairing with a detection antibody that recognizes a distinct epitope or a post-translational modification to enhance specificity for the activated kinase.
• Validate the antibody in your specific sample system, as CDK8 expression levels may vary across cell types and physiological conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to premium IVD raw materials, technical support, and consulting services—from concept to clinic. For CDK8 and other transcription-related targets, we supply validated monoclonal/polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents. Our global network also enables the sourcing of rare-target raw materials to accelerate your assay development.