Anti-CDK8 Rabbit Monoclonal Antibody for WB and ELISA - P49336

Core Product Specifications and Parameters

Biological Background: CDK8 Function and Localization

• CDK8 is a cyclin-dependent kinase and a core component of the Mediator complex, a coactivator essential for regulated transcription of nearly all RNA polymerase II-dependent genes.
• It bridges gene-specific regulatory proteins and the basal transcription machinery, scaffolding the assembly of the pre-initiation complex with RNA polymerase II and general transcription factors.
• CDK8 phosphorylates the C-terminal domain (CTD) of RNA polymerase II, which can inhibit formation of the transcription initiation complex, acting as a transcriptional repressor. Related references: PMID:10993082
• It phosphorylates CCNH, leading to down-regulation of the TFIIH complex and transcriptional repression. Related references: PMID:10993082
• Through interaction with MAML1, CDK8 hyperphosphorylates the intracellular domain of NOTCH, promoting its degradation. Related references: PMID:15546612
• CDK8 selectively modulates the interferon response by phosphorylating the STAT1 transactivation domain.
• Localized predominantly in the nucleus, CDK8 is a serine/threonine-protein kinase with activator and repressor roles, and genetic variants are linked to autism spectrum disorder and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 364–464 of human CDK8, a region that may provide specificity; consider verifying epitope uniqueness in your target system.
• For Western blotting, optimize antibody dilution using known CDK8-positive lysates (e.g., HeLa or HEK293 cells) and confirm the expected ~53 kDa band; appropriate loading controls should be included.
• For ELISA, coat with recombinant CDK8 protein or cell lysates and validate signal linearity across a concentration range; cross-reactivity with mouse CDK8 supports dual-species assays.
• Since this is a recombinant rabbit monoclonal antibody, expect high batch-to-batch reproducibility; nevertheless, re-validate performance when applying to new experimental conditions.
• Note that CDK8 is involved in dynamic phosphorylation events; consider phosphatase inhibitor cocktails during sample preparation to preserve phospho-signaling states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research teams with integrated access to premium IVD raw materials, from validated antibody pairs to rare-target reagents. For transcription and kinase pathway studies, we offer a robust supply chain including anti-CDK8 monoclonal antibodies, bulk ancillary reagents, and tailored sourcing support—streamlining your path from concept to clinic. Let us bridge the gap between your research needs and reliable raw-material procurement.