Anti-CDK6 Rabbit Polyclonal Antibody for WB - Q00534

Core Product Specifications and Parameters

Biological Background: CDK6 Function and Localization

• CDK6 (Cyclin-dependent kinase 6) is a serine/threonine-protein kinase that plays a pivotal role in cell cycle progression, promoting the G1/S transition by phosphorylating retinoblastoma protein (pRB/RB1) and nucleophosmin (NPM1).
• It forms active complexes with D-type cyclins during interphase, controlling entry into the cell cycle, and is essential for the proliferation of specific cell types, including hematopoietic and erythroid cells.
• CDK6 also regulates cell differentiation by preventing cell cycle exit in certain contexts; it is required for thymocyte development and promotes newborn neuron production in the hippocampus and subventricular zone.
• The protein localizes to the cytoplasm, nucleus, and centrosome, and is also found at cell ruffles and the cytoskeleton, indicating diverse subcellular dynamics.
• In the centrosome, CDK6 may play a role in organization during the cell cycle phases. Related references: PMID:23918663
• Tissue expression is ubiquitous, with accumulation in squamous cell carcinomas, proliferating hematopoietic progenitors, pancreatic beta-cells, and neuroblastomas; levels decrease during differentiation.
• Key post-translational modifications include phosphorylation and acetylation, and the protein is classified under keywords such as ATP-binding, phosphoprotein, and primary microcephaly disease variant.
• CDK6 delays senescence and modulates cell motility and actin cytoskeleton organization, particularly in astrocytes, contributing to cell differentiation processes.

Experimental Guidance and Technical Tips

• The antibody is raised against a synthetic peptide from residues 250–326 of human CDK6; verify epitope accessibility in your experimental system.
• For Western blotting, typical starting dilutions may range from 1:500 to 1:2000; empirical optimization is recommended.
• Include appropriate positive and negative cell/tissue lysates to confirm specificity, especially given cross-reactivity with human, mouse, and rat.
• Use standard SDS-PAGE and transfer conditions, and consider using a loading control such as β-actin or GAPDH for normalization.
• The predicted molecular weight is ~37 kDa; confirm band size against a molecular weight marker.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive pipeline of IVD raw materials, including validated CDK6 antibodies like this polyclonal, to support diagnostic and research applications in oncology, cell cycle, and neurobiology. Our global sourcing network ensures flexible supply of antibody pairs, monoclonal/polyclonal antibodies, ancillary reagents, and rare-target raw materials, streamlining your development from concept to clinic.