Anti-CDK6 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q00534

Core Product Specifications and Parameters

Biological Background: CDK6 Function and Localization

• Cyclin-dependent kinase 6 (CDK6), also known as cell division protein kinase 6 or serine/threonine-protein kinase PLSTIRE, is a member of the CMGC family of serine/threonine kinases.
• Functions as a key regulator of the cell cycle and differentiation; promotes G1/S transition by phosphorylating substrates such as pRB/RB1 and NPM1.
• Interacts with D-type cyclins during G1 to form active cyclin D-CDK6 complexes that drive progression through the restriction point and entry into S phase.
• Plays a dual role in differentiation: generally prevents proliferation and negatively regulates differentiation, yet is essential for the proliferation of specific cell types, including erythroid and hematopoietic cells.
• Required for adult neurogenesis in the dentate gyrus of the hippocampus and the subventricular zone, likely by modulating G1 length and promoting newborn neuron production.
• May play a role in centrosome organization during the cell cycle phases. Related references: PMID:23918663
• Subcellularly localized to the cytoplasm, nucleus, cell ruffles, cytoskeleton, and centrosomes, consistent with its diverse cellular functions.
• Ubiquitously expressed, with accumulation in squamous cell carcinomas, proliferating hematopoietic progenitors, pancreatic beta-cells, and neuroblastomas; expression decreases during differentiation.

Experimental Guidance and Technical Tips

• The immunogen spans residues 226–326 in the C-terminal half of human CDK6, which may allow recognition of full-length and certain splice variant forms.
• In Western blotting, expect a band at approximately 37 kDa; optimize dilution and use appropriate controls for confirming band specificity.
• For immunohistochemistry, consider using heat-induced epitope retrieval and validate staining in known positive tissues (e.g., proliferating areas) and negative controls.
• If performing immunoprecipitation, use a suitable lysis buffer that preserves protein interactions and verify capture with a control antibody.
• For immunofluorescence, permeabilize cells to detect both cytoplasmic and nuclear pools; include a blocking step to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes a one-stop platform for IVD raw materials, technical services, and consulting. For CDK6 and cell cycle research, we supply validated antibodies (including this polyclonal), matched antibody pairs, bulk ancillary reagents, and rare-target sourcing. Our integrated support streamlines development from concept to clinical application.