Anti-CDK6 Rabbit Monoclonal Antibody for WB and ELISA - Q00534

Core Product Specifications and Parameters

Biological Background: CDK6 Function and Localization

• CDK6 (Cyclin-dependent kinase 6), also known as cell division protein kinase 6 or serine/threonine-protein kinase PLSTIRE, is a member of the cyclin-dependent kinase family.
• It phosphorylates pRB/RB1 and NPM1, promoting G1/S transition and controlling cell cycle entry.
• Interacts with D-type G1 cyclins during interphase to regulate cell proliferation and differentiation; prevents myeloid differentiation by interfering with RUNX1.
• Essential for proliferation of specific cell types including erythroid, hematopoietic, and neural progenitor cells (dentate gyrus and subventricular zone).
• Promotes newborn neuron production by modulating G1 length; also involved in thymocyte development and beta-cell proliferation in pancreatic islets.
• May play a role in centrosome organization during cell cycle phases (PubMed:23918663). Related references: PMID:23918663
• Subcellularly localized to cytoplasm, nucleus, cell projections (ruffles), cytoskeleton, and centrosome.
• Expression is ubiquitous; elevated in squamous cell carcinomas, proliferating hematopoietic progenitors, beta-cells, and neuroblastomas; reduced in differentiating cells.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 227-326 of human CDK6, potentially ensuring target specificity.
• This antibody is validated for Western blot (WB) and ELISA; consider optimizing conditions for your specific samples.
• The predicted molecular weight is 37kDa; in WB experiments, bands may be observed near this size, but post-translational modifications could influence migration.
• Cross-reactivity is confirmed with human and rat species; for other species, assess sequence homology and validate.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material partner, CamelBio supplies top-tier reagents like this anti-CDK6 rabbit monoclonal antibody for cell cycle and oncology research. We support your entire development pipeline with validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents, plus custom sourcing for rare targets. Connect with CamelBio to streamline your diagnostic development from concept to clinic.