Anti-CDK5RAP3 (C53/LZAP) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96JB5

Core Product Specifications and Parameters

Biological Background: CDK5RAP3 Function and Localization

• Acts as a substrate adapter of E3 ligase complexes mediating ufmylation, the covalent attachment of UFM1 to substrate proteins, playing a role in ribosome recycling and reticulophagy. Related references: PMID:23152784, PMID:30635284, PMID:32851973
• Within the UREL complex, promotes mono-ufmylation of the 60S ribosomal subunit protein RPL26 at Lys-134, facilitating release and recycling of post-termination ribosomes from the endoplasmic reticulum membrane. Related references: PMID:38383785, PMID:38383789, PMID:36121123
• Participates in reticulophagy (ER-phagy) during ER stress by mediating ufmylation of proteins such as CYB5R3, targeting them for lysosomal degradation. Related references: PMID:36543799
• Negatively regulates NF-kappa-B-mediated transcription through control of RELA phosphorylation, influencing inflammatory and immune responses. Related references: PMID:17785205, PMID:20228063
• Regulates mitotic G2/M transition and DNA damage checkpoint; interacts with CDKN2A/ARF and MDM2 to induce p53 stabilization and G1 cell cycle arrest. Related references: PMID:15790566, PMID:19223857, PMID:16173922
• Localizes to the endoplasmic reticulum membrane, cytoplasm, nucleus, and centrosome, consistent with its diverse roles in protein modification, transcription, and cell cycle.
• Ubiquitously expressed in tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas, with isoform 3 enriched in kidney, liver, skeletal muscle, and placenta.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-300) targets the N-terminal region of CDK5RAP3, conserved across human, mouse, and rat, supporting reliable cross-reactivity in these species.
• For western blot (WB), a specific band near 57 kDa is expected; consider using total protein normalization or housekeeping proteins for loading control validation.
• In immunofluorescence (IF/ICC), anticipate detection in the cytoplasm, nucleus, and ER membrane; co-localization with organelle-specific markers may help confirm subcellular distribution.
• The polyclonal nature may yield robust signal in ELISA; however, each new assay format should be validated with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers and research labs by offering this anti-CDK5RAP3 rabbit polyclonal antibody, a tool for investigating ufmylation pathways, ribosome quality control, and cell cycle checkpoints. Beyond single antibodies, we supply validated antibody pairs, bulk ancillary reagents, and customized raw-material sourcing for rare or emerging targets. Our integrated supply chain and technical consultancy help accelerate your development from concept to clinical application.