Anti-CDK5RAP2 Polyclonal Antibody for WB, ELISA - Q96SN8

Core Product Specifications and Parameters

Biological Background: CDK5RAP2 Function and Localization

• CDK5RAP2 (Cep215/C48/MCPH3) is a ~215 kDa centrosomal protein encoded by the CDK5RAP2 gene, which undergoes alternative splicing.
• Acts as a potential regulator of CDK5 activity via interaction with CDK5R1. Related references: PMID:15164053
• Functions as a negative regulator of centriole disengagement, maintaining centriole engagement and cohesion, and is involved in regulating mitotic spindle orientation.
• Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of BUBR1 and MAD2. Related references: PMID:19282672
• Together with EB1/MAPRE1, promotes microtubule polymerization, bundle formation, growth and dynamics at the plus ends. Related references: PMID:18042621 PMID:17959831 PMID:19553473
• Regulates centrosomal maturation by recruiting the gamma-tubulin ring complex (gTuRC) onto centrosomes. Related references: PMID:18042621 PMID:17959831 PMID:26485573
• Contributes to microtubule nucleation and extension from the centrosome to the cell periphery in complex with PDE4DIP, MAPRE1, and AKAP9, and is required for AKAP9 recruitment to centrosomes. Related references: PMID:29162697
• Localizes to the cytoplasm, centrosome, microtubule organizing center, and Golgi apparatus; widely expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas; mutations are linked to primary microcephaly type 3 (MCPH3).

Experimental Guidance and Technical Tips

• For Western blotting, consider using reducing and denaturing sample preparation due to the large molecular weight (~215 kDa) of CDK5RAP2; include appropriate positive and negative control lysates from human cell lines known to express the target.
• For ELISA, the recombinant immunogen fragment (aa 1744-1893) may serve as a coating standard; validate antibody specificity against full-length protein and relevant controls.
• Given the role of CDK5RAP2 in centrosome biology, immunofluorescence may be a complementary application; optimize fixation and permeabilization conditions for centrosomal antigens.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, providing diagnostic manufacturers and research institutes with access to high-quality antibodies for targets like CDK5RAP2, which is essential for cell cycle and neurodevelopment studies. Our offerings include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets, all designed to streamline your assay development and procurement processes.