Anti-CDK5RAP1 Polyclonal Antibody for WB, IHC-P, ELISA - Q96SZ6

Core Product Specifications and Parameters

Biological Background: CDK5RAP1 Function and Localization

• CDK5RAP1 (also known as C42, CGI-05, HSPC167, C20orf34) is a mitochondrial methylthiotransferase that modifies four mitochondrial tRNAs (Ser(UCN), Phe, Tyr, Trp) by converting N6-(dimethylallyl)adenosine (i6A) to 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37, which is crucial for translation accuracy. Related references: PMID:22422838 PMID:25738458 PMID:28981754

• The enzyme specifically inhibits CDK5 activation by its regulator CDK5R1 (p35), implicating a role in neuronal signaling pathways. Related references: PMID:11882646

• CDK5RAP1 belongs to the radical S-adenosyl-L-methionine (SAM) superfamily and contains a conserved 4Fe-4S cluster essential for its methylthiotransferase activity.

• Subcellular localization is predominantly mitochondrial, consistent with its function in mitochondrial tRNA modification and protein synthesis.

• Tissue expression is widespread, with highest levels in brain, placenta, and testis; notably, it is expressed in neurons of the central nervous system.

• Essential for mitochondrial protein synthesis and respiratory chain function; pathological mutations are associated with mitochondrial disease. Related references: PMID:25738458

• The protein exists in multiple alternatively spliced isoforms and contains a mitochondrial transit peptide for import.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight is approximately 68 kDa; use reducing conditions and optimize loading and antibody dilution for your specific sample.
• In immunohistochemistry (IHC-P), antigen retrieval may be required; consider testing a range of antibody dilutions to minimize background.
• For ELISA, the antibody can be employed in direct, indirect, or sandwich formats; pair with a suitable detection system.
• Cross-reactivity with mouse and rat allows application in rodent models; validate detection specificity in relevant tissue or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing solutions for IVD raw materials, covering every stage from concept to clinical application. This anti-CDK5RAP1 polyclonal antibody supports research into mitochondrial biology, tRNA modification, and translational regulation. With validated antibody pairs, bulk ancillary reagents, and rare target sourcing capabilities, CamelBio enables diagnostic manufacturers and research laboratories to accelerate biomarker discovery and assay development.