Anti-CDK5R1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15078

Core Product Specifications and Parameters

Biological Background: CDK5R1 Function and Localization

• CDK5R1 (cyclin-dependent kinase 5 activator 1) encodes the regulatory subunit p35 and its truncated form p25, both serving as neuron-specific activators of cyclin-dependent kinase 5 (CDK5).
• The p35/CDK5 complex is essential for proper neuronal development, including neurite outgrowth and cortical lamination, and mediates EFNA1-EPHA4 signaling to regulate dendritic spine morphogenesis.
• CDK5R1 participates in circadian rhythm regulation by interacting with CLOCK; the p35/CDK5 complex phosphorylates CLOCK at Thr-451 and Thr-461, modulating the transcriptional activity of the CLOCK-BMAL1 heterodimer and affecting its stability and subcellular distribution.
• The protein localizes to multiple subcellular compartments: cell membrane, neuron projections, cytoplasm (perinuclear region), and nucleus, reflecting its diverse roles in signaling and regulation.
• Expression of CDK5R1 is highly specific to brain and neuronal tissues.
• Key post-translational modifications include myristoylation (lipoprotein), phosphorylation, and ubiquitination (Ubl conjugation); a three-dimensional structure has been determined.

Experimental Guidance and Technical Tips

• For Western blotting, CDK5R1 (p35) is predicted at ~34 kDa; a calpain-generated p25 cleavage product (~25 kDa) may also be detected. Use appropriate molecular weight markers and consider validating band identity with knockdown or knockout controls.
• In immunofluorescence, optimize cell permeabilization and fixation for detection in neuronal cells, as CDK5R1 localizes to membranes, neurites, and perinuclear regions. Co-staining with compartment markers can aid localization studies.
• As a polyclonal antibody, batch-to-batch consistency should be verified; titrate the antibody for each new lot in your specific experimental setup.
• For ELISA, evaluate the antibody as a capture or detection reagent with appropriate standards; cross-reactivity with mouse and rat CDK5R1 homologs suggests utility for multi-species assays, but validate in the sample matrix of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material solutions, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents. For CDK5R1-related neuroscience research and diagnostic development, we supply high-quality reagents backed by technical support across the entire workflow—from concept to clinic. Partner with us for reliable, one-stop sourcing of rare targets and custom raw materials.