Anti-CDK5 Rabbit Polyclonal Antibody (KO Validated) for WB, ELISA - Q00535

Core Product Specifications and Parameters

Biological Background: CDK5 Function and Localization

• CDK5 is a proline-directed serine/threonine kinase critical for neuronal cell cycle exit, differentiation, and survival. It is activated by p35/CDK5R1 and p39/CDK5R2, predominantly in postmitotic neurons.
• It phosphorylates a wide array of substrates, including cytoskeletal proteins (MAPT/TAU, MAP1B, MAP2), transcription factors (MEF2A, p53/TP53), and regulators of vesicle trafficking (SYN1, DNM1, AMPH), thereby modulating neurite growth, synaptic plasticity, and neurotransmission.
• CDK5 plays dual roles in cell death: it promotes survival via BCL2 and STAT3 activation, but under stress conditions (genotoxic, oxidative) it can induce apoptosis through p53/TP53 phosphorylation and stabilization, and aberrant activation by p25 leads to neuronal death.
• Subcellular localization includes cytoplasm, nucleus, cell membrane, and neuronal projections such as growth cones and postsynaptic densities, reflecting its diverse functions in signal transduction and cytoskeletal dynamics.
• Keywords associated with CDK5 include ATP-binding, kinase, transferase, phosphoprotein, neurodegeneration, neurogenesis, and biological rhythms, highlighting its involvement in critical cellular and neurological processes.
• Tissue specificity: Ubiquitously expressed, with accumulation in cortical neurons (PubMed:17009320, PubMed:19693690). Also expressed in testis, skeletal muscle, colon, bone marrow, and ovary. Related references: PMID:17009320 PMID:19693690
• Post-translational modifications: Acetylation and phosphorylation regulate its activity and subcellular distribution, contributing to its tight control in normal physiology and disease.

Experimental Guidance and Technical Tips

• The immunogen, corresponding to the kinase domain (aa 170-289) of human CDK5, is conserved across human, mouse, and rat, supporting the reported cross-reactivity; consider sequencing the target region in other species for extension.
• For Western blot, a primary band is expected at ~33 kDa, but note that post-translational modifications or splice variants may produce additional bands; include a positive control (e.g., brain lysate) and perform blocking optimization.
• When using for ELISA, consider coating antigen directly or using a capture antibody pair; validate linearity and sensitivity in the target sample matrix.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; include a reference standard in each run.
• For subcellular localization studies, note CDK5’s presence in cytoplasm, nucleus, and membrane compartments; cellular fractionation or co-staining with organelle markers may aid in result interpretation.

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