Anti-CDK5 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q00535

Core Product Specifications and Parameters

Biological Background: CDK5 Function and Localization

• CDK5 is a proline-directed serine/threonine-protein kinase, also known as Cell division protein kinase 5, Cyclin-dependent-like kinase 5, and Tau protein kinase II catalytic subunit.
• It plays a critical role in neuronal cell cycle arrest and differentiation, and is implicated in apoptotic cell death associated with neurodegenerative diseases.
• The kinase is activated by binding to regulatory subunits CDK5R1 (p35) and CDK5R2 (p39), which are predominantly expressed in postmitotic neurons.
• CDK5 phosphorylates numerous substrates involved in neuronal development, including MAPT/TAU, histone H1, p53/TP53, and synaptic proteins like SYN1 and DNM1, regulating processes such as axon guidance, synaptogenesis, and neurotransmission.
• It localizes to various cellular compartments including the cytoplasm, nucleus, cell membrane, lamellipodia, growth cones, and postsynaptic density, consistent with its diverse functions.
• CDK5 is ubiquitously expressed, with prominent accumulation in cortical neurons. It is found in testis, skeletal muscle, colon, bone marrow, and ovary. Related references: PMID:17009320 PMID:19693690
• Phosphorylation of certain substrates (e.g., MEF2A, p53/TP53, APEX1) by CDK5 mediates apoptotic pathways, while phosphorylation of BCL2 and STAT3 promotes cell survival, highlighting its dual role in neuronal fate.
• The protein is classified under serine/threonine-protein kinases and features ATP-binding and transferase domains. Post-translational modifications include acetylation and phosphorylation (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human CDK5 protein (aa 1-292). For Western blot, expect a band near 33 kDa; however, consider that post-translational modifications or alternative splicing may yield additional bands.
• Suitable applications include WB, IF/ICC, IP, and ELISA. For immunoprecipitation, optimize lysis buffer conditions and include appropriate controls to validate specificity in your sample system.
• The antibody has been KO validated, indicating suitability for studies requiring knockout control; nonetheless, confirm specificity under your own experimental conditions.
• Given cross-reactivity with mouse and rat, this antibody is applicable for rodent models; species-specific validation is recommended.
• For IF/ICC, follow standard fixation and permeabilization protocols; CDK5 expression is enriched in neurons, so consider using neuronal cell lines or primary cultures.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, offering validated antibody pairs, high-quality monoclonal and polyclonal antibodies, and bulk ancillary reagents. For CDK5-focused research in neurodegeneration, cell cycle, and neuronal development, CamelBio provides reliable one-stop access from concept to clinic. Explore our portfolio to accelerate your diagnostic and research projects with robust, ready-to-use raw materials.