Anti-CDK5 Rabbit Monoclonal Antibody [KO Validated] for WB and ELISA - Q00535

Core Product Specifications and Parameters

Biological Background: CDK5 Function and Localization

• CDK5 (cyclin-dependent kinase 5), also known as cell division protein kinase 5, PSSALRE, LIS7, and tau protein kinase II catalytic subunit, is a proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest, differentiation, and may trigger abortive cell cycle re-entry in neuronal apoptosis.
• It interacts with D1 and D3-type G1 cyclins and phosphorylates numerous substrates including SRC, NOS3, VIM/vimentin, p35/CDK5R1, MAPT/TAU, MAP1B, histone H1, p53/TP53, and others, regulating neuronal survival, migration, differentiation, axonal growth, synaptogenesis, and synaptic plasticity.
• CDK5 is activated by binding to CDK5R1 (p35) and CDK5R2 (p39), particularly in postmitotic neurons; aberrant cleavage of p35 to p25 leads to deregulated activity associated with neurodegeneration.
• Subcellularly, CDK5 localizes to the cytoplasm, nucleus, cell membrane, perikaryon, lamellipodia, growth cones, postsynaptic densities, and synapses, reflecting its diverse functional roles.
• It is ubiquitously expressed but accumulates in cortical neurons (at protein level), and is also found in testis, skeletal muscle, colon, bone marrow, and ovary. Related references: PMID:17009320, PMID:19693690
• Key biological processes regulated by CDK5 include apoptosis, circadian rhythms, cytoskeletal reorganization, endocytosis, exocytosis, and negative regulation of Wnt/beta-catenin signaling.
• Given its involvement in lissencephaly, neurodegeneration, and neurogenesis, CDK5 is a critical target for neuroscience and cell cycle research.

Experimental Guidance and Technical Tips

• The recombinant immunogen corresponding to amino acids 92-289 of human CDK5 suggests that the antibody recognizes an epitope within this region; consider using the full-length protein or fragments for specificity validation.
• For Western blot, a band around 33 kDa is expected; ensure proper protein loading and use appropriate molecular weight markers.
• Given KO validation, this antibody may exhibit high specificity; however, users should confirm performance in their own sample preparations and experimental conditions.
• Cross-reactivity with mouse and rat allows application across common model organisms; validate tissue-specific expression patterns in relevant species.
• In ELISA, optimize coating antigen concentration and detection system to achieve optimal signal-to-noise ratio.
• For neuronal studies, consider enriching for cytoplasmic or synaptic fractions to enhance CDK5 detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as your comprehensive one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and research laboratories with a seamless bridge from concept to clinic. Our curated portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. For your CDK5-related research and assay development, CamelBio ensures reliable supply and technical support to accelerate your neuroscience and cell cycle projects.