Anti-CDK4 Rabbit PolymAb® for WB, IP, IF/ICC, IF-P, IHC-P, ELISA - P11802

Core Product Specifications and Parameters

Biological Background: CDK4 Function and Localization

• CDK4 (Cyclin-dependent kinase 4), also known as Cell division protein kinase 4 or PSK-J3, is a serine/threonine-protein kinase encoded by the CDK4 gene in humans.
• As the catalytic subunit of cyclin D-CDK4 complexes, CDK4 phosphorylates and inhibits retinoblastoma (RB) protein family members, notably RB1, during the G1/S transition of the cell cycle.
• Phosphorylation of RB1 by CDK4 releases E2F transcription factors, enabling transcription of genes required for S-phase entry and cell cycle progression.
• CDK4 also phosphorylates SMAD3 in a cell-cycle-dependent manner, repressing its transcriptional activity and integrating mitogenic and antimitogenic signals.
• Subcellular localization includes cytoplasm, nucleus, and nuclear membrane; translocation into the nucleus is facilitated by the ternary cyclin D/CDK4/CDKN1B complex.
• Keywords from UniProt highlight its roles in ATP binding, cell cycle, cell division, and its regulation by acetylation and phosphorylation; disease variants are linked to cancer.
• The predicted molecular weight is approximately 34 kDa (UniProt: 33.7 kDa), and alternative splicing may generate additional isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 204-303 of human CDK4. Ensure the target epitope is accessible in your assay format; denaturing and reducing conditions may be required for Western blotting.
• For Western blot (WB), a band around 34 kDa is expected in lysates from human, mouse, or rat cell lines; confirm band specificity using appropriate positive and negative controls.
• In immunoprecipitation (IP), optimize antibody-to-lysate ratios and include a relevant control antibody to assess pull-down efficiency.
• For immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), validate staining patterns against known CDK4 subcellular distributions (nuclear and cytoplasmic) in proliferating cells.
• ELISA applications may require pairing with a suitable detection antibody and standard curves using recombinant CDK4 protein.
• Always perform preliminary titration experiments to determine optimal working concentrations for your specific samples and protocols.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, to streamline your diagnostic and research workflows.
• With expertise in cell cycle-related targets, CamelBio supports your CDK4 projects from concept to clinic, providing one-stop access to rare target raw-material sourcing and technical consulting.
• Whether you are developing a CDK4-based IVD kit or studying cell proliferation, rely on CamelBio for consistent quality and reliable supply of critical reagents.