Anti-CDK4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P11802

Core Product Specifications and Parameters

Biological Background: CDK4 Function and Localization

• CDK4 (Cyclin-dependent kinase 4), also known as Cell division protein kinase 4 or PSK-J3, is a serine/threonine-protein kinase encoded by the CDK4 gene. It is a key component of cyclin D-CDK4 complexes that regulate cell cycle progression.
• As part of the cyclin D-CDK4 complex, CDK4 phosphorylates and inhibits members of the retinoblastoma (RB) protein family, including RB1, during the G1/S transition. This phosphorylation leads to the dissociation of the transcription factor E2F from RB/E2F complexes, enabling transcription of E2F target genes required for progression through G1 phase.
• CDK4 hypophosphorylates RB1 in early G1 phase and serves as a major integrator of various mitogenic and antimitogenic signals through its activity in the cyclin D-CDK4 complex.
• CDK4 also phosphorylates SMAD3 in a cell-cycle-dependent manner, repressing its transcriptional activity, highlighting its role beyond RB regulation.
• CDK4 forms a ternary complex with cyclin D and CDKN1B (p27), which is required for nuclear translocation and activity of the cyclin D-CDK4 complex.
• Subcellular localization includes the cytoplasm, nucleus, and nucleus membrane, reflecting its dynamic role in cell cycle regulation.
• Keywords from UniProt indicate involvement in acetylation, alternative splicing, ATP-binding, cell cycle, cell division, and disease variants; it is a phosphoprotein linked to cancer-related pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200-303 of human CDK4, a region proximal to the C-terminus. This epitope may influence recognition in certain applications; consider validating in the specific experimental context.
• For Western blot (WB), a band around 34 kDa is expected. Validate in human and mouse cell lysates expressing CDK4, as cross-reactivity is reported for both species.
• For immunofluorescence (IF/ICC), explore both cytoplasmic and nuclear staining patterns, consistent with CDK4’s subcellular localization. Include appropriate fixation and permeabilization steps.
• Immunoprecipitation (IP) with this polyclonal antibody may enrich CDK4 complexes; consider verifying co-precipitation of cyclin D or CDKN1B if studying the ternary complex.
• As a polyclonal antibody, batch-to-batch consistency should be verified for quantitative assays like ELISA. Perform titration experiments to determine optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to IVD raw materials like this CDK4 antibody, supporting cell cycle and oncology-related assay development. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare targets to streamline your diagnostic workflows.