Anti-CDK2AP1 Rabbit Polyclonal Antibody for WB, ELISA - O14519

Core Product Specifications and Parameters

Biological Background: CDK2AP1 Function and Localization

• CDK2AP1 (Cyclin-dependent kinase 2-associated protein 1), also known as Deleted in oral cancer 1 (DOC-1) or putative oral cancer suppressor, functions as a tumor suppressor.
• It acts as an inhibitor of cyclin-dependent kinase CDK2, thereby regulating cell cycle progression.
• CDK2AP1 is a component of the histone deacetylase NuRD complex, participating in chromatin remodeling and transcriptional regulation. Related references: PMID:16428440 PMID:20523938 PMID:28977666
• This protein localizes to the nucleus and associates with chromosomes, consistent with its roles in transcription regulation and chromatin modification.
• Post‑translational modifications include phosphorylation, and the protein contains disulfide bonds that may influence its structure and interactions.
• Alternative splicing generates multiple isoforms, adding functional diversity.

Experimental Guidance and Technical Tips

• For Western blot, consider using a starting dilution range optimized for polyclonal antibodies (e.g., 1:500–1:2000) and validate with positive and negative controls expressing CDK2AP1.
• The immunogen corresponds to the N‑terminal region (aa 1–115) of human CDK2AP1; ensure your detection system recognizes epitopes in this domain.
• Because CDK2AP1 has a calculated molecular weight of ~12 kDa, use a high‑percentage SDS‑PAGE gel (e.g., 12–15%) and a suitable membrane with small pore size (0.2 µm) for efficient transfer.
• For ELISA, titrate the antibody against the recombinant immunogen or native protein to determine optimal coating and detection conditions.
• Cross‑reactivity has been confirmed for human; when working with other species, verify sequence homology and perform pilot assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, mono‑ and polyclonal antibodies, and bulk ancillary reagents. This CDK2AP1 Rabbit pAb, targeting a key cell‑cycle and chromatin regulatory protein, exemplifies our commitment to supporting cancer and epigenetics research. Through CamelBio, diagnostic developers and researchers gain streamlined access to high‑quality reagents, technical expertise, and custom sourcing solutions—helping you advance from concept to clinic with confidence.