Anti-CDK20 Polyclonal Antibody for IHC-P and ELISA - Q8IZL9

Core Product Specifications and Parameters

Biological Background: CDK20 Function and Localization

• Cyclin-dependent kinase 20 (CDK20) is also known as CDK-activating kinase p42 (CAK-kinase p42), cell cycle-related kinase (CCRK), and CDK-activating kinase p42.
• CDK20 is a serine/threonine-protein kinase that plays a critical role in cell growth and cell cycle progression.
• CDK20 activates CDK2 by phosphorylation at Thr-160, thereby promoting cell cycle control.
• CDK20 is required for high-level Sonic Hedgehog (Shh) signaling responses in the developing neural tube, operating together with TBC1D32 to coordinate primary cilium membrane and axoneme assembly, ensuring proper GLI2 activation.
• The protein localizes to the nucleus, cytoplasm, and cell projections, notably the primary cilium.
• CDK20 belongs to the cyclin-dependent kinase family and contains functional domains for ATP binding and nucleotide binding.
• Alternative splicing of CDK20 produces multiple isoforms, though specific isoform functions remain to be fully characterized.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 200-300 of human CDK20, suggesting that this antibody may recognize epitopes in the central region of the protein.
• For immunohistochemistry (IHC-P), consider optimizing antigen retrieval conditions and testing in tissues known to express CDK20, such as those with active Shh signaling or ciliated cells.
• In ELISA applications, validate assay conditions using recombinant CDK20 or cell lysates from human or rat samples to confirm binding specificity.
• Given the reported cross-reactivity with rat, this antibody may be suitable for studies in rat models; however, users should verify reactivity in their specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and research institutions, offering one-stop access to high-quality IVD raw materials including validated antibodies, recombinant proteins, and ancillary reagents. This CDK20 antibody supports research in cell cycle regulation, ciliogenesis, and developmental signaling pathways. For bulk orders, custom formulations, or sourcing of rare target reagents, our team is ready to assist with tailored solutions.