Anti-CDK2 Rabbit Polyclonal Antibody [KO Validated] for WB, IHC-P, ELISA - P24941

Core Product Specifications and Parameters

Biological Background: CDK2 Function and Localization

• CDK2 (cyclin-dependent kinase 2), also known as p33 protein kinase, is a serine/threonine-protein kinase encoded by the CDK2 gene (alternative name CDKN2). It is essential for meiosis but dispensable for mitosis. Related references: PMID:10499802, PMID:10884347, PMID:10995386

• CDK2 drives G1-S transition by binding cyclin E to promote the E2F transcriptional program and DNA synthesis; later, it pairs with cyclin A2 to drive S-G2 progression and mitotic entry via cyclin B/CDK1 activation. Related references: PMID:18372919, PMID:19238148, PMID:19561645

• CDK2 triggers centrosome duplication and DNA replication by phosphorylating NPM1, causing its dissociation from unduplicated centrosomes. Related references: PMID:11051553

• In DNA damage response, CDK2 regulates homologous recombination repair by phosphorylating BRCA2 (low in S phase, high as cells approach mitosis) and NBN for telomere repair. Related references: PMID:15800615, PMID:20195506, PMID:21319273, PMID:28216226

• Key phosphorylation targets include RB1 (disrupts E2F1 binding), MYC (prevents Ras-induced senescence), NPAT (activates histone gene transcription), and EZH2 (maintains H3K27me3 epigenetic silencing). Related references: PMID:10499802, PMID:10995386, PMID:10995387, PMID:19966300, PMID:20935635

• CDK2 localizes to the cytoplasm, especially the centrosome and endosomes, and to the nucleus including Cajal bodies, shuttling between compartments during the cell cycle.

• CDK2 plays a crucial role in embryonic stem cells by orchestrating a balance between proliferation, cell death, and DNA repair. Related references: PMID:18372919, PMID:19238148, PMID:19561645

• CDK2 is subject to multiple post-translational modifications including phosphorylation, acetylation, and S-nitrosylation (as annotated by UniProt keywords), which modulate its activity and interactions.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning residues 200–298 of human CDK2. For Western blotting, expect bands around the predicted molecular weights of ~30 kDa and ~34 kDa; use lysates from cell lines such as HeLa or MCF7 and validate with appropriate controls.
• For IHC-P applications, antigen retrieval conditions may need optimization; confirm staining specificity by comparing with negative controls or knockout tissue sections.
• Given its reactivity with human, mouse, and rat samples, the antibody is suitable for cross-species studies; however, epitope conservation should be verified for non-human targets.
• Since CDK2 is a cell cycle-dependent kinase, consider cell cycle synchronization or treatment with cell cycle inhibitors to enrich for active CDK2 detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, from monoclonal/polyclonal antibodies to ancillary reagents and rare target development. Our CDK2 polyclonal antibody, validated for WB, IHC-P, and ELISA, serves cell cycle and oncology research, and we can assist with bulk supply, custom antibody development, and technical consultation to accelerate your assay development.