Anti-CDK2 (Phospho-T160) Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P24941

Core Product Specifications and Parameters

Biological Background: CDK2 Function and Localization

• CDK2 is a cyclin-dependent kinase that orchestrates cell cycle progression, primarily governing the G1-S and G2-M transitions.
• It triggers the duplication of centrosomes and DNA, thereby ensuring proper mitotic entry. Related references: PMID:11051553
• At the G1-S transition, cyclin E-bound CDK2 phosphorylates NPAT, which activates histone gene transcription necessary for S phase. Related references: PMID:10995386, PMID:10995387
• CDK2 phosphorylates the retinoblastoma protein RB1, disrupting RB1-E2F1 complexes and releasing E2F1 to transcribe S-phase genes. Related references: PMID:10499802
• In the nucleus, CDK2 participates in DNA damage repair by regulating BRCA2 phosphorylation; reduced CDK2 activity promotes homologous recombination. Related references: PMID:15800615, PMID:20195506, PMID:21319273
• The kinase shuttles between the cytoplasm (centrosome, endosome, cytoskeleton) and the nucleus (Cajal body), and its activity is controlled by phosphorylation at Thr-160 by the CDK-activating kinase complex, as well as by acetylation and S-nitrosylation. Related references: PMID:41100585

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Thr-160; confirm phosphorylation specificity using phosphatase treatment or non-phosphopeptide competition.
• For western blot (WB), expect a band at approximately 34 kDa; validate detection in cell lines with high CDK2 expression (e.g., HeLa or Jurkat).
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), anticipate nuclear and centrosomal staining, with cell-cycle-dependent localization.
• Cross-reacts with mouse and rat CDK2; verify performance in the relevant species and tissue context.
• For ELISA applications, optimize coating conditions and use appropriate phospho-peptide controls to ensure assay specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-Phospho-CDK2-T160 monoclonal antibody addresses critical needs in cell cycle and oncology research, and is part of our extensive portfolio of phospho-specific antibodies. Beyond individual reagents, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—streamlining your assay development and procurement.