Anti-CDK18 Polyclonal Antibody for WB and ELISA - Q07002

Core Product Specifications and Parameters

Biological Background: CDK18 Function and Localization

• CDK18 (also known as PCTK3, PCTAIRE, PCTAIRE3) is a member of the cyclin-dependent kinase family, functioning as a serine/threonine-protein kinase.
• It may play a role in signal transduction cascades in terminally differentiated cells.
• The gene gives rise to at least two isoforms through alternative splicing: isoform 1 is widely expressed, while isoform 2 expression is limited to several subcortical nuclei of the basal ganglia and the spinal cord.
• CDK18 is an ATP-binding protein and a phosphoprotein, indicating regulation by phosphorylation.
• Key functional motifs and annotations include: ATP-binding, nucleotide-binding, kinase activity, and phosphoprotein status.
• The predicted molecular weight is approximately 54 kDa, consistent with the observed protein weight in Western blot.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant protein covering amino acids 373-474 of human CDK18, corresponding to the C-terminal region. This region may confer isoform specificity.
• For Western blot, consider using standard denaturing and reducing conditions with a band expected at ~54 kDa. Validate the antibody in your specific sample system, as expression levels and post-translational modifications may vary.
• For ELISA, ensure appropriate antigen coating and include positive and negative controls. Cross-reactivity with human and mouse has been reported, but testing in other species is recommended if needed.
• As CDK18 is a kinase potentially involved in phosphorylation cascades, the antibody may detect both phosphorylated and unphosphorylated forms unless epitope masking occurs. Phospho-specific detection should be independently validated.

CamelBio: Your One-Stop Sourcing Bridge

For diagnostic manufacturers and research laboratories requiring high-quality IVD raw materials, CamelBio provides streamlined access to validated antibodies, antigens, and ancillary reagents. Our portfolio includes well-characterized antibodies like this anti-CDK18 polyclonal antibody, supporting signal transduction and kinase research. As a one-stop partner, we assist with bulk sourcing, custom reagent development, and technical consulting, helping you move from concept to clinic efficiently.