Anti-CDK16 Polyclonal Antibody for IF/ICC, ELISA - Q00536

Core Product Specifications and Parameters

Biological Background: CDK16 Function and Localization

• Cyclin-dependent kinase 16 (CDK16), also known as PCTAIRE1, PCTK1, or serine/threonine-protein kinase PCTAIRE-1, is a member of the CDK family encoded by the CDK16 gene.
• CDK16 plays a role in vesicle-mediated transport and exocytosis; it regulates GH1 release by brain neurons through phosphorylation of NSF, thereby modulating NSF oligomerization (By similarity).
• The kinase is required for normal spermatogenesis and regulates neuron differentiation and dendrite development (By similarity).
• CDK16 is involved in insulin secretion in response to blood glucose changes and can phosphorylate cyclin-Y (CCNY) at Ser-336 in vitro.
• Subcellularly, CDK16 localizes to the cytoplasm, cytoplasmic vesicles, secretory vesicles, cell membrane, and synaptic compartments (synapse/synaptosome).
• At the protein level, CDK16 is detected in pancreas islets, with additional expression in brain and pancreas tissues.
• Keywords for CDK16 include kinase, transferase, phosphoprotein, alternative splicing, and reference proteome, underscoring its regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 277–496) of human CDK16, which may target conserved epitopes; consider epitope conservation if using other species.
• For IF/ICC, optimize fixation and permeabilization protocols, as CDK16 associates with multiple membrane compartments.
• In ELISA, validate linearity and specificity using the recombinant immunogen or cell lysates from CDK16-expressing tissues (brain, pancreas).
• CDK16 is a low-abundance kinase; signal amplification or enrichment strategies may be beneficial for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined sourcing solution for IVD raw materials. For kinases like CDK16 involved in exocytosis and neuronal function, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to support your assay development and research needs.