Anti-CDK12 Monoclonal Antibody for IHC-P, ELISA - Q9NYV4

Core Product Specifications and Parameters

Biological Background: CDK12 Function and Localization

• CDK12 (Cyclin-dependent kinase 12) is a member of the CDK family of serine/threonine kinases, also known as Cdc2-related kinase arginine/serine-rich (CrkRS) and cell division protein kinase 12.
• It plays a central role in transcription elongation by phosphorylating the C-terminal domain (CTD) of RNA polymerase II, preferentially targeting Ser-5 in pre-phosphorylated CTD repeats.
• CDK12 is required for the expression of DNA repair genes, thereby maintaining genomic stability.
• The kinase also regulates mRNA splicing, possibly through phosphorylation of splicing factors such as SRSF1/SF2.
• Subcellularly, CDK12 localizes to the nucleus and nuclear speckles, consistent with its role in transcription and splicing.
• It is widely expressed across human tissues.
• Alternative splicing and chromosomal rearrangements of CDK12 have been observed, with implications in cancer biology.
• Post-translational modifications including phosphorylation and ubiquitination modulate its activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 420-516 of human CDK12, a region within the kinase domain, ensuring specific recognition of the catalytic region.
• For IHC-P, consider optimizing antigen retrieval and antibody dilution using known positive control tissues.
• For ELISA, coat with recombinant CDK12 protein or peptide immunogen to establish standard curves.
• The antibody shows cross-reactivity with human, mouse, and rat, enabling multi-species studies; however, validation in the specific experimental system is recommended.
• As a rabbit monoclonal antibody, it offers high specificity and lot-to-lot consistency for reproducible assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing validated antibodies like this anti-CDK12 monoclonal antibody to support transcription and oncology research. We offer a comprehensive range of products including optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to streamline your diagnostic development pipeline.