Anti-CDK11B Polyclonal Antibody for WB, IF/ICC, ELISA - P21127

Core Product Specifications and Parameters

Biological Background: CDK11B Function and Localization

• CDK11B is a cyclin-dependent kinase that functions as a critical regulator of both transcription and pre-mRNA splicing. Related references: PMID:12501247, PMID:18216018, PMID:32367068
• It phosphorylates the splicing factor SF3B1 at key residues, promoting its association with U5 and U6 snRNAs and facilitating spliceosome assembly. Related references: PMID:36104565, PMID:38059508
• CDK11B phosphorylates Ser-2 of the C-terminal domain (CTD) of RNA polymerase II (POLR2A), directly coupling transcription to downstream RNA processing events. Related references: PMID:32367068, PMID:40858114
• It is specifically required for replication-dependent histone gene transcription, controlling transcriptional elongation and recruitment of 3'-end processing factors at histone loci. Related references: PMID:32367068
• CDK11B functions upstream of CDK9 in a transcription checkpoint that regulates promoter-proximal pausing by RNA polymerase II. Related references: PMID:40858114
• The kinase is activated by the CDK-activating kinase (CAK) complex comprising CDK7, cyclin-H (CCNH), and MAT1. Related references: PMID:41100585
• Alternatively spliced isoforms exist: one is expressed in a non-cell cycle-dependent manner, while another isoform is specifically expressed during G2-M phases and promotes centromeric transcription to maintain centromeric cohesion during mitosis. Related references: PMID:12082095, PMID:2217177, PMID:24436071, PMID:38019613
• CDK11B localizes to the nucleus, chromosomes, centromeres, and cytoplasm, reflecting its diverse roles in transcription, splicing, and cell cycle regulation.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-130) of human CDK11B; this region may be conserved across isoforms. Consider validating isoform specificity in your target samples using appropriate controls (e.g., isoform-specific knockdown or overexpression).
• For Western blot, a band at approximately 93 kDa is expected for full-length CDK11B. Use whole-cell or nuclear extracts and standard SDS-PAGE conditions. Optimize antibody dilution for your specific samples.
• In immunofluorescence/immunocytochemistry (IF/ICC), nuclear and centromeric staining patterns are anticipated. Include fixation/permeabilization optimization and peptide blocking controls to confirm signal specificity.
• This antibody cross-reacts with human and mouse CDK11B as per the manufacturer’s data. If using other species, verify reactivity experimentally due to potential sequence variability.
• For ELISA applications, the antibody can be used as a capture or detection reagent; titrate in the relevant assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers, laboratories, and research institutes with streamlined access to validated raw materials, including this anti-CDK11B polyclonal antibody for transcription and cell cycle studies. Our portfolio encompasses optimized monoclonal and polyclonal antibodies, antibody pairs, and bulk ancillary reagents to support diagnostic development. From rare target sourcing to comprehensive technical consultation, CamelBio accelerates your path from concept to clinic.