Anti-CDK10 Rabbit Polyclonal Antibody for WB, ELISA - Q15131

Core Product Specifications and Parameters

Biological Background: CDK10 Function and Localization

• CDK10 (Cyclin-dependent kinase 10) is a serine/threonine-protein kinase, also known as cell division protein kinase 10 or PISSLRE, and belongs to the cyclin-dependent kinase family (keywords: Kinase, Serine/threonine-protein kinase, Transferase).
• It is an ATP-binding phosphoprotein that plays a role in cell cycle regulation and actin cytoskeleton organization (keywords: ATP-binding, Nucleotide-binding, Phosphoprotein).
• CDK10 phosphorylates the transcription factor ETS2 in vitro and controls its proteasomal degradation in cells, thereby modulating transcriptional activity. Related references: PMID:24218572
• It acts as a negative regulator of ciliogenesis by phosphorylating PKN2 and promoting RhoA signaling, linking the kinase to primary cilium dynamics. Related references: PMID:27104747
• The protein localizes primarily to the cytoplasm, with specific enrichment at the cytoskeleton and cilium basal body (subcellular locations: Cytoplasm, cytoskeleton, cilium basal body).
• CDK10 is involved in cilium biogenesis and degradation, as indicated by the keyword "Cilium biogenesis/degradation" and its association with cell projections.
• The UniProt record reports alternative splicing, suggesting multiple transcript variants may exist (keyword: Alternative splicing), and the reference proteome status confirms its comprehensive annotation.
• Its molecular weight is approximately 41 kDa (calculated from sequence: 41038 Da), consistent with the product’s observed band size.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-283) of human CDK10, which may yield reactivity against multiple isoforms if alternative splicing preserves this domain.
• For WB, consider using reducing conditions and standard SDS-PAGE protocols; the predicted molecular weight is 41 kDa, but post-translational modifications may shift the apparent band.
• Antibody specificity can be assessed by comparing signals in human versus mouse lysates, given the stated cross-reactivity; validate in the relevant sample system with appropriate controls.
• In ELISA, the recombinant protein immunogen serves as a positive control; performance may vary with native protein conformation.
• This polyclonal antibody is suitable for detecting CDK10 in cell models where the kinase is expressed endogenously (e.g., for experiments involving cytoskeletal dynamics or ciliogenesis).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of high-quality IVD raw materials for diagnostic developers, research laboratories, and academic institutes. This anti-CDK10 polyclonal antibody can be integrated into workflows studying cell cycle regulation, cytoskeletal organization, or ciliopathy-related targets. As your one-stop sourcing partner, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized assistance in sourcing rare-target raw materials — all backed by technical and supply chain expertise to accelerate your projects from concept to clinic.