Anti-CDK1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P06493

Core Product Specifications and Parameters

Biological Background: CDK1 Function and Localization

• CDK1 (Cyclin-dependent kinase 1) is the catalytic subunit of the M-phase promoting factor (MPF), essential for G2-M transition and mitotic onset. It phosphorylates numerous substrates to drive centrosome separation, Golgi dynamics, nuclear envelope breakdown, and chromosome condensation. Related references: PMID:16407259 PMID:16933150 PMID:17459720 PMID:18480403 PMID:20360007
• Its activity is tightly regulated by cyclin binding and reversible phosphorylation. During G2, CDC25 phosphatases remove inhibitory phosphorylations to activate CDK1, while WEE1/PKMYT1 kinases inhibit it. Once chromosomes align at metaphase, CDK1 inactivation by WEE1/PKMYT1 allows mitotic exit and cytokinesis. Related references: PMID:18480403 PMID:20360007 PMID:2188730
• CDK1 localizes to multiple subcellular compartments, including the nucleus, cytoplasm, mitochondria, and mitotic structures such as centrosomes and the spindle, reflecting its diverse roles in cell division.
• It phosphorylates lamins (LMNA, LMNB1, LMNB2) at mitotic entry, triggering nuclear lamina disassembly and nuclear envelope breakdown. Related references: PMID:2188730 PMID:2344612 PMID:37788673
• Beyond mitosis, CDK1 regulates apoptosis, circadian rhythms, and gene silencing through phosphorylation of targets such as FOXO1, NR1D1, and EZH2. It also acts as a receptor for hepatitis C virus (HCV) entry into hepatocytes.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, which modulate its activity and stability. CDK1 is also a key player in DNA damage checkpoints, where PKR/EIF2AK2- and WEE1-mediated phosphorylation halts the cell cycle to allow repair.
• Tissue expression is widespread, with elevated levels in proliferating cells and a notable association with breast cancer tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 198-297 of human CDK1, a region containing key regulatory phosphorylation sites. Ensure that the epitope is not masked by post-translational modifications in your sample.
• For Western blot, expect a band around 34 kDa. Because CDK1 is cell-cycle regulated, consider synchronizing cells or enriching for mitotic populations to enhance signal.
• When using for immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution. Inclusion of appropriate positive and negative controls (e.g., untreated vs. cell-cycle arrested samples) is recommended.
• Validate cross-reactivity in your specific mouse or rat tissues/strains, as sequence homology may affect detection.
• This antibody has been tested in WB, IHC-P, IF/ICC, IP, and ELISA; consider performing pilot experiments to confirm suitability for your particular assay and target species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with validated IVD raw materials, including optimized antibodies for cell cycle and oncology research. Whether you need validated antibody pairs, bulk ancillary reagents, or assistance sourcing rare-target raw materials, our comprehensive supply chain supports every stage from concept to clinic. Leverage our expertise to accelerate your development of CDK1-based diagnostic assays.