Anti-CDK1 Polyclonal Antibody for WB, IHC-P, ELISA - P06493

Core Product Specifications and Parameters

Biological Background: CDK1 Function and Localization

• CDK1 (CDC2) is a serine/threonine-protein kinase that drives G2-M transition and mitotic entry by associating with multiple cyclins, regulating centrosome cycle and chromosome segregation. Related references: PMID:16407259, PMID:16933150, PMID:17459720
• Catalyzes lamin (LMNA, LMNB1, LMNB2) phosphorylation at mitosis onset, promoting nuclear envelope breakdown; also phosphorylates histones, cytoskeletal proteins, and transcriptional regulators. Related references: PMID:2188730, PMID:2344612, PMID:37788673
• Activity is tightly controlled by phosphorylation: activated by CDC25 phosphatases and the CDK7-cyclin H-MAT1 (CAK) complex; inactivated by WEE1 and PKMYT1 kinases. Related references: PMID:18480403, PMID:20360007, PMID:2188730, PMID:41100585
• DNA damage triggers CDK1 inhibition via PKR/EIF2AK2 and WEE1 to enforce G2 checkpoint arrest; subsequent WIP1-dependent reactivation allows cell cycle resumption after repair. Related references: PMID:20360007, PMID:20395957
• Subcellular localization includes nucleus, cytoplasm, mitochondria, centrosome, and mitotic spindle, reflecting its dynamic roles in cell division.
• Tissue-specific expression is notable in breast cancer tissues; CDK1 also serves as a host receptor for hepatitis C virus (HCV) entry.
• Key regulatory modifications include phosphorylation, acetylation, and ubiquitination; the protein is implicated in apoptosis, circadian rhythms, and viral infection.

Experimental Guidance and Technical Tips

• The full-length immunogen (aa 1–297) may allow recognition of multiple CDK1 conformations; pre‑adsorption or competition assays can confirm specificity if needed.
• For WB, consider testing both total and phospho‑specific signals due to dynamic CDK1 phosphorylation during the cell cycle; synchronize cells for clearer mitotic profiles.
• For IHC‑P, antigen retrieval conditions may require optimization; validate staining in known positive tissues (e.g., tonsil, breast carcinoma).
• Species cross‑reactivity with mouse and rat is reported; confirm endogenous detection in the relevant model systems prior to quantitative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers and research groups with validated IVD raw materials, including high‑quality antibodies like this anti‑CDK1 polyclonal for cell cycle and oncology applications. We offer one‑stop access to antibody pairs, bulk ancillary reagents, and sourcing support for rare targets—accelerating your journey from concept to clinic.