Anti-CDH4 Rabbit Polyclonal Antibody for WB, ELISA - P55283

Core Product Specifications and Parameters

Biological Background: CDH4 Function and Localization

• CDH4 encodes Cadherin-4, also known as retinal cadherin (R-cadherin), a classical cadherin cell adhesion molecule.
• Cadherins are calcium-dependent transmembrane proteins that mediate homophilic cell-cell adhesion, contributing to tissue organization and the sorting of heterogeneous cell types.
• CDH4 is a member of the cadherin superfamily, characterized by extracellular cadherin repeat domains, a single-pass transmembrane helix, and a cytoplasmic tail.
• Alternative splicing of CDH4 produces multiple isoforms. The protein undergoes signal peptide cleavage and glycosylation, with a predicted molecular mass of approximately 100 kDa.
• Subcellularly, CDH4 localizes to the plasma membrane, where it participates in adherens junctions.
• Tissue expression is predominantly in the brain, but it is also detected in other tissues, with a suggested role in retinal development.
• The cytoplasmic domain may interact with catenins, linking cadherins to the actin cytoskeleton, which is essential for stable cell adhesion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 757–916) of human CDH4, which is part of the cytoplasmic domain. This region is likely well-conserved across species, consistent with the reported cross-reactivity.
• For Western blotting, a band around 100 kDa is expected. Consider reducing and denaturing conditions, and validate in cell lines or tissues known to express CDH4 (e.g., brain, retinal cells).
• For ELISA, the antibody may be suitable for detecting recombinant or native CDH4. Optimization of coating and blocking conditions is recommended.
• As with all polyclonal antibodies, lot-to-lot variability may occur. Inclusion of appropriate positive and negative controls is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers, clinical laboratories, and research institutions with a comprehensive portfolio of raw materials, technical services, and consulting—from early concept to clinical application. This anti-CDH4 antibody is part of our cell adhesion and developmental biology offering, and we can further provide validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and custom sourcing for rare target raw materials. Contact our team to streamline your next diagnostic or research project.