Anti-CDH11 Polyclonal Antibody for WB, IF/ICC, ELISA - P55287

Core Product Specifications and Parameters

Biological Background: Cadherin-11 Function and Localization

• Cadherin-11 (CDH11), also known as osteoblast cadherin (OB-cadherin) and OSF-4, is a member of the cadherin family of calcium-dependent cell adhesion proteins.
• It mediates homophilic cell-cell adhesion, contributing to the sorting of heterogeneous cell types, and is required for proper focal adhesion assembly. Related references: PMID:33811546
• CDH11 is involved in the regulation of cell migration. Related references: PMID:33811546
• Localized to the cell membrane, CDH11 is expressed mainly in the brain, with detection in neuroblasts and embryonic facial mesenchyme, including palatal, dental, and ocular structures. Related references: PMID:33811546
• Post-translational modifications include glycosylation, phosphorylation, and cleavage on pair of basic residues.
• Keywords associated with CDH11 encompass alternative splicing, calcium binding, cell adhesion, membrane, metal-binding, and disease variants (deafness, intellectual disability) linked to chromosomal rearrangements.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the extracellular domain of human CDH11; the resulting polyclonal antibody may recognize multiple epitopes, enhancing detection sensitivity across applications.
• For Western blotting, a band at approximately 88 kDa is expected; consider that post-translational modifications may cause slight molecular weight shifts.
• When using immunofluorescence, validate in relevant cell lines (e.g., osteoblasts or neuronal cells) to confirm membrane localization and minimize background; mouse cross-reactivity allows testing in mouse models.
• For ELISA, optimize coating antigen and antibody dilutions empirically, keeping in mind the calcium-dependent nature of cadherin interactions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research institutions, offering this anti-CDH11 antibody alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Specializing in cell adhesion and developmental biology targets, CamelBio streamlines procurement for bone and brain research applications, and can assist in sourcing rare-target raw materials to support your diagnostic or assay development pipeline.