Anti-CDCP1 Rabbit Polyclonal Antibody for WB, ELISA - Q9H5V8

Core Product Specifications and Parameters

Biological Background: CDCP1 Function and Localization

• Protein identity: CDCP1 (CUB domain-containing protein 1) is a membrane glycoprotein also known as gp140, TRASK, SIMA135, and CD318. It belongs to the tetraspanin web and plays roles in tumor progression and metastasis.
• Cell adhesion and matrix interaction: CDCP1 is involved in cell adhesion and cell matrix association, regulating the balance between anchorage-dependent processes and migration or proliferation versus differentiation, likely through its phosphorylation state.
• Disease relevance: CDCP1 is proposed as a novel marker for leukemia diagnosis and for identifying immature hematopoietic stem cell subsets. It is up‑regulated in various human tumor cell lines, as well as in colorectal cancer, breast carcinoma, and lung cancer.
• Expression pattern: Highly expressed in mitotic cells, with low levels during interphase. In normal tissues, highest expression is observed in skeletal muscle and colon, with lower levels in kidney, small intestine, placenta, and lung.
• Subcellular localization: CDCP1 is found at the cell membrane and can also be secreted, consistent with its roles in cell‑surface interactions and signaling.
• Post‑translational modifications: The protein is a glycoprotein and phosphoprotein, containing disulfide bonds that may stabilize its extracellular domain structure.
• Isoform diversity: Alternative splicing generates multiple isoforms, which may contribute to functional diversification in different cell types and conditions.

Experimental Guidance and Technical Tips

• The immunogen used (amino acids 30–343 of human CDCP1) encompasses a large extracellular portion, making this polyclonal antibody suitable for detecting multiple epitopes on the native protein. For western blot (WB), consider using appropriate positive and negative control lysates (e.g., from cells with known CDCP1 expression levels) and validate the detected band at the expected molecular weight (~93 kDa) in the relevant sample system.
• For ELISA applications, it is advisable to optimize coating and detection conditions, and to include appropriate reference antigens and controls to assess specificity and sensitivity in your assay setup.
• Given the reported cross‑reactivity with mouse CDCP1, this antibody may be useful for comparative studies in mouse models, but cross‑reactive performance should be confirmed under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering one‑stop access to validated antibodies, optimized monoclonal and polyclonal reagents, bulk ancillary reagents, and sourcing of rare‑target raw materials. Our CDCP1 antibody supports research in cell adhesion, tumor progression, and leukemia diagnostics, and we can also assist you with complementary reagents, technical services, and supply chain support to streamline your assay development from concept to clinic.