Anti-CDCP1 Monoclonal Antibody for FC - Q9H5V8

Core Product Specifications and Parameters

Biological Background: CDCP1 Function and Localization

• CDCP1 (CUB domain-containing protein 1), also designated CD318, TRASK, or SIMA135, is a transmembrane glycoprotein and a component of the tetraspanin web, which is implicated in tumor progression and metastasis.
• The protein is believed to mediate cell adhesion and cell-matrix association, with its phosphorylation potentially regulating the balance between anchorage and migration, or between proliferation and differentiation.
• CDCP1 localizes predominantly to the cell membrane and can also be secreted; it is highly expressed in mitotic cells, skeletal muscle, and colon, with lower levels in kidney, small intestine, placenta, and lung.
• Upregulation of CDCP1 is observed in various human tumor cell lines, including colorectal, breast, and lung cancers, and it has been proposed as a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets.
• The protein undergoes alternative splicing, producing isoforms with predicted molecular weights of 37 kDa, 72 kDa, and 92 kDa.
• Post-translational modifications include glycosylation and phosphorylation, as supported by proteomics identification and direct protein sequencing.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein covering the extracellular domain (amino acids 30–666), suggesting that the antibody recognizes an extracellular epitope, making it suitable for live-cell staining in flow cytometry.
• Since the antibody is already conjugated to , no secondary detection reagent is needed; optimize the antibody dilution for your specific cell type and confirm CDCP1 expression beforehand (e.g., using tumor cell lines known to upregulate CDCP1).
• Include appropriate isotype controls and viability dyes in your flow cytometry panel to ensure specific staining, and validate the antibody in the relevant sample system under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, bringing together validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing capabilities. This anti-CDCP1 antibody fits into cancer biomarker research and leukemia diagnostic development, and CamelBio can support your entire workflow from early-stage discovery through clinical assay manufacturing with tailored procurement solutions and technical consultation.