Anti-CDCA8 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q53HL2

Core Product Specifications and Parameters

Biological Background: CDCA8 Function and Localization

• CDCA8 (Borealin) is a core component of the chromosomal passenger complex (CPC), essential for accurate chromosome segregation and cytokinesis during mitosis.
• It interacts with Aurora B kinase, INCENP, and Survivin to regulate kinetochore-microtubule attachments and error correction.
• Major effector of TTK kinase in controlling attachment-error-correction and chromosome alignment.
• Required for chromatin-induced microtubule stabilization and spindle assembly.
• Localizes to the nucleus and nucleolus in interphase, then relocalizes to the centromere and mitotic spindle during mitosis.
• Phosphorylation at multiple sites regulates its CPC function and cell cycle progression.
• Subject to ubiquitination; identified as a phosphoprotein in proteomics studies.
• CDCA8 is conserved across species and is listed under keywords: 3D-structure, cell cycle, cell division, centromere, chromosome, cytoplasm, cytoskeleton, isopeptide bond, mitosis, nucleus, phosphoprotein, proteomics identification, reference proteome, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 280 amino acids of human CDCA8; the antibody may recognize multiple isoforms or post-translationally modified forms.
• For western blotting, consider testing a range of dilutions and lysates from human, mouse, or rat cell lines; validate band specificity via siRNA knockdown or overexpression.
• In immunohistochemistry (paraffin), antigen retrieval protocols and antibody titration should be optimized; nuclear and mitotic cell staining can serve as positive controls.
• For ELISA applications, pairing with a validated detection antibody may improve sensitivity; cross-reactivity with other CPC components should be assessed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For CDCA8-related assay development—whether for cell cycle, cancer diagnostics, or mitotic regulation studies—CamelBio provides optimized polyclonal and monoclonal antibody options, bulk supply capabilities, and expert consultancy to accelerate your project from concept to clinic.