Anti-CDCA8 Rabbit Polyclonal Antibody for WB, ELISA - Q53HL2

Core Product Specifications and Parameters

Biological Background: CDCA8 Function and Localization

• CDCA8, also known as Borealin or Dasra-B, is a key component of the chromosomal passenger complex (CPC), essential for proper chromosome segregation during mitosis.
• The CPC localizes to centromeres and ensures correct chromosome alignment, attachment-error correction, and spindle assembly, with CDCA8 acting as a major effector of the TTK kinase in these processes.
• CDCA8 is a cell-cycle-regulated phosphoprotein that undergoes isopeptide bond formation and ubiquitin-like conjugation, modifications critical for its mitotic functions.
• Subcellularly, CDCA8 dynamically localizes to the nucleolus during interphase and moves to the centromere, spindle, and cytoplasm during mitosis.
• Together with other CPC subunits (AURKB, INCENP, Survivin), CDCA8 facilitates chromatin-induced microtubule stabilization and maintains genomic stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (amino acids 1‑124) of human CDCA8, which is unique to this protein; consider verifying sequence conservation across species if cross‑reactivity is critical.
• For Western blot, the predicted molecular weight is ~31 kDa; optimize denaturing conditions and antibody dilution for your specific lysate and detection system.
• Polyclonal antibodies may exhibit lot‑to‑lot variability; validate new lots in the intended application and sample type.
• Although ELISA is listed as an application, determine appropriate coating concentrations and blocking strategies empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to supply high‑quality IVD raw materials, including validated antibodies like this anti‑CDCA8 polyclonal antibody for mitosis and cell‑cycle studies. Beyond individual reagents, we offer one‑stop access to optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, supporting your projects from concept to clinic.