Anti-CDCA5 Polyclonal Antibody for WB - Q96FF9

Core Product Specifications and Parameters

Biological Background: CDCA5 Function and Localization

• CDCA5, also known as sororin and p35, is a cell division cycle-associated protein that functions as a key regulator of sister chromatid cohesion during mitosis.
• It stabilizes the association of the cohesin complex with chromatin, possibly by antagonizing the action of WAPL, which promotes cohesin dissociation.
• Proper cohesion ensures accurate chromosome partitioning in both mitotic and meiotic cells and plays a critical role in DNA repair processes.
• CDCA5 is specifically required for efficient DNA double-stranded break repair, highlighting its importance in maintaining genomic integrity.
• Subcellularly, CDCA5 localizes to the nucleus and associates with chromosomes, but can also be detected in the cytoplasm.
• Post-translational modifications include phosphorylation and ubiquitin-like conjugation (Ubl conjugation), as indicated by proteomics studies.
• The protein has a predicted molecular weight of approximately 27.6 kDa, consistent with the observed ~28 kDa band in western blotting.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fragment spanning amino acids 70–150 of human CDCA5; this region may be critical for recognizing endogenous sororin in WB.
• For western blotting, a band around 28 kDa is expected in lysates from human, mouse, or rat cells; validate in your specific sample system.
• In ELISA applications, consider using the recombinant immunogen or full-length protein as a positive control.
• Since this is a polyclonal antibody, lot-to-lot variability may occur; we recommend initial titration and consistent validation in your assay.
• For immunofluorescence or other untested applications, the antibody may still perform; however, empirical optimization is advised.

CamelBio: Your One-Stop Sourcing Bridge

Whether you're developing a cohesive diagnostic assay or studying cell cycle checkpoints, CamelBio provides reliable access to IVD raw materials like this anti-CDCA5 polyclonal antibody. As your sourcing partner from concept to clinic, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and even help source rare targets such as sororin. Reach out to explore how our procurement solutions can accelerate your project's timeline.