Anti-CDCA3 Rabbit Polyclonal Antibody for WB, ELISA - Q99618

Core Product Specifications and Parameters

Biological Background: CDCA3 Function and Localization

• CDCA3, also known as GRCC8 and TOME-1, is a 28998 Da F-box-like protein.
• It is required for entry into mitosis by participating in E3 ligase complexes that mediate ubiquitination and degradation of WEE1 kinase at the G2/M phase transition (By similarity).
• CDCA3 localizes to the cytoplasm and cytosol.
• The protein is classified under cell cycle, cell division, and mitosis keywords.
• It is subject to phosphorylation and ubiquitin-like (Ubl) conjugation, indicating potential post-translational regulation.
• As a reference proteome member, CDCA3 has been identified through proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 100–C-terminus) of human CDCA3; antibody reactivity may be influenced by post-translational modifications in this region.
• For Western blot, an expected band around 29 kDa should be observed; confirm specificity using appropriate controls such as knockdown or knockout lysates.
• The antibody is suitable for ELISA; optimize coating antigen and detection conditions for your specific assay format.
• Since CDCA3 expression peaks at G2/M, synchronizing cells in mitosis may enhance detection.
• Cross-reactivity is reported with mouse CDCA3; verify in mouse samples to confirm suitability for mouse model studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti-CDCA3 product, and bulk ancillary reagents. For researchers investigating cell cycle control and mitotic entry, we provide comprehensive support from antibody procurement to technical consulting. Contact us to streamline your raw material sourcing and accelerate your diagnostic or research development.