Anti-CDCA3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q99618

Core Product Specifications and Parameters

Biological Background: CDCA3 Function and Localization

• CDCA3, also known as GRCC8 or TOME-1, is an F-box-like protein that functions as a substrate recognition component of E3 ubiquitin ligase complexes.
• It plays a critical role in the G2/M checkpoint by targeting WEE1 kinase for ubiquitination and proteasomal degradation, thereby promoting mitotic entry (By similarity).
• Subcellularly, CDCA3 is localized to the cytoplasm and cytosol.
• The protein is a phosphoprotein and participates in ubiquitin conjugation pathways, indicating regulation by post-translational modifications.
• Classified under keywords such as cell cycle, cell division, and mitosis, CDCA3 is integral to cell proliferation control.

Experimental Guidance and Technical Tips

• For Western blotting, use whole-cell lysates from human cell lines; an expected band near 29 kDa may be observed. Ensure sample preparation minimizes phosphatase activity if phosphorylation-dependent forms are of interest.
• In immunohistochemistry (paraffin), antigen retrieval may be necessary; validate staining in human tissue sections known to express CDCA3, such as proliferative tissues.
• For ELISA, the antibody can be used to detect recombinant CDCA3 or peptide fragments; consider testing various coating conditions to optimize sensitivity.
• Since CDCA3 expression is cell cycle-regulated, consider synchronizing cells at the G2/M boundary to enhance detection if baseline levels are low.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-CDCA3 antibody supports cell cycle and oncology research applications, and our team can assist in sourcing related rare targets or developing customized assays. Contact us to streamline your raw material procurement from concept to clinic.