Anti-Cdc6 Polyclonal Antibody for WB - Q99741

Core Product Specifications and Parameters

Biological Background: Cdc6 Function and Localization

• Cdc6 (Cell division control protein 6 homolog) is a DNA replication factor essential for the initiation of DNA replication.
• It loads the minichromosome maintenance (MCM) complex onto replication origins, licensing them for DNA synthesis.
• Cdc6 also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
• The protein is mainly localized in the nucleus but can also be found in the cytoplasm.
• Post-translational modifications include phosphorylation and ubiquitination, which regulate its activity and stability.
• Cdc6 is an ATP-binding protein, and its nucleotide-binding activity is critical for its function.
• Mutations in CDC6 are associated with Meier-Gorlin syndrome 5 (MGORS5), a disorder characterized by dwarfism and other developmental defects.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody covers nearly the full-length Cdc6 protein (aa 1-560), suggesting the polyclonal antibody may recognize multiple epitopes.
• For Western blot, consider using whole cell lysates from human cell lines (e.g., HeLa, HEK293) and loading 20–30 µg of protein. Expect a band around 63 kDa.
• For ELISA, the antibody can be tested as both capture and detection reagent; perform titration to determine optimal working concentrations.
• Always validate the antibody’s specificity in your specific experimental context, as cross-reactivity with other species or related proteins cannot be ruled out.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-Cdc6 polyclonal antibody is ideal for Western blot and ELISA applications in DNA replication and cell cycle research. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing services to support every stage of your diagnostic development, from concept to clinic.